Skip to main content
NCBI home page
Plant Physiology logoLink to Plant Physiology
. 1974 May;53(5):779–781. doi: 10.1104/pp.53.5.779

Rapid Increase in Adenosine 5′-Triphosphate during Early Wheat Embryo Germination 1

Ralph L Obendorf a,2, Abraham Marcus a
PMCID: PMC541446  PMID: 16658790

Abstract

The ATP content of isolated wheat (Triticum aestivum L. var. Polk) embryos increases 5-fold during the first 30 minutes and 10-fold during the first hour of germination to 80% of maximum. The ATP level remains at approximately 800 nanomoles per gram of tissue during the next 15 hours. ADP, AMP, and total adenosine phosphates decrease between 1 and 6.5 hours, while adenylate energy charge increases from 0.6 to 0.8 and remains constant. The rapid increase in ATP during imbibition is consistent with the energy requirement for polyribosome formation and protein synthesis during the first hours of germination. A method for determining nanomole quantities of ATP in tissue extracts by isotopic dilution of γ-32P-ATP in the hexokinase reaction is outlined.

Full text

PDF
779

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Atkinson D. E. Regulation of enzyme function. Annu Rev Microbiol. 1969;23:47–68. doi: 10.1146/annurev.mi.23.100169.000403. [DOI] [PubMed] [Google Scholar]
  2. BROWN E. G. CHANGES IN THE FREE NUCLEOTIDE PATTERN OF PEA SEEDS IN RELATION TO GERMINATION. Biochem J. 1965 May;95:509–514. doi: 10.1042/bj0950509. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. CONWAY T. W., LIPMANN F. CHARACTERIZATION OF A RIBOSOME-LINKED GUANOSINE TRIPHOSPHATASE IN ESCHERICHIA COLI EXTRACTS. Proc Natl Acad Sci U S A. 1964 Dec;52:1462–1469. doi: 10.1073/pnas.52.6.1462. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Cashel M., Lazzarini R. A., Kalbacher B. An improved method for thin-layer chromatography of nucleotide mixtures containing 32P-labelled orthophosphate. J Chromatogr. 1969 Mar 11;40(1):103–109. doi: 10.1016/s0021-9673(01)96624-5. [DOI] [PubMed] [Google Scholar]
  5. Chapman A. G., Fall L., Atkinson D. E. Adenylate energy charge in Escherichia coli during growth and starvation. J Bacteriol. 1971 Dec;108(3):1072–1086. doi: 10.1128/jb.108.3.1072-1086.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Ching T. M. Adenosine triphosphate content and seed vigor. Plant Physiol. 1973 Feb;51(2):400–402. doi: 10.1104/pp.51.2.400. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Ching T. M., Ching K. K. Content of adenosine phosphates and adenylate energy charge in germinating ponderosa pine seeds. Plant Physiol. 1972 Nov;50(5):536–540. doi: 10.1104/pp.50.5.536. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Crane R. K. Use of Charcoal to Separate Mixtures of Inorganic, Ester, and Nucleotide Phosphates. Science. 1958 Feb 7;127(3293):285–286. doi: 10.1126/science.127.3293.285. [DOI] [PubMed] [Google Scholar]
  9. Freudenberg H., Mager J. Studies on the mechanism of the inhibition of protein synthesis induced by intracellular ATP depletion. Biochim Biophys Acta. 1971 Mar 25;232(3):537–555. doi: 10.1016/0005-2787(71)90608-3. [DOI] [PubMed] [Google Scholar]
  10. JOHNSTON F. B., STERN H. Mass isolation of viable wheat embryos. Nature. 1957 Jan 19;179(4551):160–161. doi: 10.1038/179160b0. [DOI] [PubMed] [Google Scholar]
  11. Marcus A., Feeley J. Ribosome activation and polysome formation in vitro: requirement for ATP. Proc Natl Acad Sci U S A. 1966 Dec;56(6):1770–1777. doi: 10.1073/pnas.56.6.1770. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Marcus A., Feeley J., Volcani T. Protein Synthesis in Imbibed Seeds III. Kinetics of Amino Acid Incorporation Ribosome Activation, and Polysome Formation. Plant Physiol. 1966 Sep;41(7):1167–1172. doi: 10.1104/pp.41.7.1167. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Marcus A. Seed germination and the capacity for protein synthesis. Symp Soc Exp Biol. 1969;23:143–160. [PubMed] [Google Scholar]
  14. Weeks D. P., Marcus A. Preformed messenger of quiescent wheat embryos. Biochim Biophys Acta. 1971 Apr 8;232(4):671–684. doi: 10.1016/0005-2787(71)90759-3. [DOI] [PubMed] [Google Scholar]

Articles from Plant Physiology are provided here courtesy of Oxford University Press

RESOURCES