Abstract
Treatment of slices of young pea leaves (Pisum sativum) with μM solutions of α-chlorallyl diethyldithiocarbamate, dichloroallyl diisopropylthiocarbamate, or S-ethyldipropylthiocarbamate resulted in inhibition of incorporation of [1-14C]acetate into C31 alkane and C31 secondary alcohol, very little effect on the synthesis of C26 and C28 fatty alcohols, and an accumulation of 14C in shorter chain cuticular lipids, particularly C22 acid. Higher concentrations of the thiocarbamates caused inhibition of synthesis of C26 and C28 fatty alcohols and an accumulation of label in C22 acid. Further increase in thiocarbamate concentration resulted in inhibition of C22 acid synthesis also. The three thiocarbamates at μM concentration also inhibited incorporation of [1-14C]stearic acid specifically into C31 alkane and C31 secondary alcohol. These results suggest that thiocarbamates reduce cuticular lipid formation by a concentration-dependent inhibition of the various chain-elongating enzyme systems.
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