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Expression and purification of LYG1. (A) Nucleotide sequence and amino acid sequence of human LYG1. The boxes indicate the signal peptide predicted by SignalP 4.0. (B) Expression profile of LYG1 in multiple human tissues and immune cells analyzed by PCR and real-time PCR. (C) Verification of the secretion pathway for LYG1 using the BFA blocking assay. (D) Pure rhLYG1 analysis by SDS-PAGE.
