There are errors in Table 1. A computational error caused incorrect gene length values to be applied to the parasite RPKM calculations, resulting in changes in the ranking of which parasite genes were most highly expressed by RNA sequencing. A corrected Table 1 is provided here.
Table 1. Top 20 L. braziliensis genes expressed in detectable-positive lesions as ranked by RPKM.
| Gene Description (IDs) | L. braziliensis RPKM |
|---|---|
| amastin-like surface protein, putative (LbrM.08.1060/08.300/08.1130) | 38295 |
| L-ribulokinase, putative (LbrM.35.0100) | 11184 |
| amastin-like surface protein, putative (LbrM.18.0460/08.0310/08.290) | 7270 |
| histone H2B (LbrM.09.1400) | 6390 |
| amastin-like surface protein, putative (LbrM.08.1100/20.4300) | 6164 |
| kinesin, putative (pseudogene) (LbrM.20.1090) | 5013 |
| unspecified product (LbrM.24.1400) | 4973 |
| tagatose-6-phosphate kinase-like protein (LbrM.02.0030) | 4232 |
| amastin-like protein (LbrM.08.1030) | 3867 |
| histone H1 (LbrM.18.1510) | 3827 |
| amastin-like surface protein, putative (LbrM.20.0790) | 3778 |
| hypothetical protein, conserved (LbrM.20.3230) | 3753 |
| amastin-like protein (LbrM.08.0670) | 3727 |
| heat-shock protein hsp70, putative (LbrM.28.2990) | 3676 |
| beta tubulin (LbrM.21.2150) | 3620 |
| amastin-like surface protein (LbrM.18.0470) | 3567 |
| histone H2A (LbrM.21.1140) | 3342 |
| amastin-like surface protein, putative (LbrM.20.1080) | 3321 |
| histone H2A, putative (LbrM.21.1160) | 3192 |
| histone H1, putative (LbrM.27.1290) | 3145 |
Reference
- 1.Christensen SM, Dillon LAL, Carvalho LP, Passos S, Novais FO, Hughitt VK, et al. (2016) Meta-transcriptome Profiling of the Human-Leishmania braziliensis Cutaneous Lesion. PLoS Negl Trop Dis 10(9): e0004992 doi:10.1371/journal.pntd.0004992 [DOI] [PMC free article] [PubMed] [Google Scholar]