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. 1975 Jan;55(1):59–63. doi: 10.1104/pp.55.1.59

Kinetics and Energetics of Light-driven Chloroplast Glutamine Synthesis 1

Cary A Mitchell a,2, C Ralph Stocking a
PMCID: PMC541550  PMID: 16659028

Abstract

Chloroplasts contain the enzyme glutamine synthetase. Formation of glutamine by isolated chloroplasts is light-dependent and requires an intact outer envelope. Addition of exogenous glutamic acid, as well as nitrogen donors such as nitrite or ammonium, stimulate the synthesis of this amide. Photosynthetic generation of ATP satisfies the light requirement of glutamine synthesis. The process is supported by cyclic as well as noncyclic photophosphorylation.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Arnon D. I. COPPER ENZYMES IN ISOLATED CHLOROPLASTS. POLYPHENOLOXIDASE IN BETA VULGARIS. Plant Physiol. 1949 Jan;24(1):1–15. doi: 10.1104/pp.24.1.1. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Arnon D. I., Tsujimoto H. Y., McSwain B. D. Ferredoxin and photosynthetic phosphorylation. Nature. 1967 May 6;214(5088):562–566. doi: 10.1038/214562a0. [DOI] [PubMed] [Google Scholar]
  3. BASSHAM J. A., MORAWIECKA B., KIRK M. PROTEIN SYNTHESIS DURING PHOTOSYNTHESIS. Biochim Biophys Acta. 1964 Sep 4;90:542–552. doi: 10.1016/0304-4165(64)90233-8. [DOI] [PubMed] [Google Scholar]
  4. Givan C. V., Givan A. L., Leech R. M. Photoreduction of alpha-Ketoglutarate to Glutamate by Vicia faba Chloroplasts. Plant Physiol. 1970 May;45(5):624–630. doi: 10.1104/pp.45.5.624. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. HEBER U. Protein synthesis in chloroplasts during photosynthesis. Nature. 1962 Jul 7;195:91–92. doi: 10.1038/195091a0. [DOI] [PubMed] [Google Scholar]
  6. Heber U., Pon N. G., Heber M. Localization of Carboxydismutase & Triosephosphate Dehydrogenases in Chloroplasts. Plant Physiol. 1963 May;38(3):355–360. doi: 10.1104/pp.38.3.355. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Heldt H. W., Sauer F. The inner membrane of the chloroplast envelope as the site of specific metabolite transport. Biochim Biophys Acta. 1971 Apr 6;234(1):83–91. doi: 10.1016/0005-2728(71)90133-2. [DOI] [PubMed] [Google Scholar]
  8. Jacobson B. S., Stumpf P. K. Fat metabolism in higher plants. LV. Acetate uptake and accumulation by class I and II chloroplasts from Spinacia oleracea. Arch Biochem Biophys. 1972 Dec;153(2):656–663. doi: 10.1016/0003-9861(72)90384-0. [DOI] [PubMed] [Google Scholar]
  9. Jensen R. G., Bassham J. A. Photosynthesis by isolated chloroplasts. Proc Natl Acad Sci U S A. 1966 Oct;56(4):1095–1101. doi: 10.1073/pnas.56.4.1095. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Kanazawa T., Kirk M. R., Bassham J. A. Regulatory effects of ammonia on carbon metabolism in photosynthesizing Chlorella pyrenoidosa. Biochim Biophys Acta. 1970 Jun 30;205(3):401–408. doi: 10.1016/0005-2728(70)90106-4. [DOI] [PubMed] [Google Scholar]
  11. Kirk P. R., Leech R. M. Amino Acid Biosynthesis by Isolated Chloroplasts during Photosynthesis. Plant Physiol. 1972 Aug;50(2):228–234. doi: 10.1104/pp.50.2.228. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Magalhaes A. C., Neyra C. A., Hageman R. H. Nitrite assimilation and amino nitrogen synthesis in isolated spinach chloroplasts. Plant Physiol. 1974 Mar;53(3):411–415. doi: 10.1104/pp.53.3.411. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Prusiner S., Milner L. A rapid radioactive assay for glutamine synthetase, glutaminase, asparagine synthetase, and asparaginase. Anal Biochem. 1970 Oct;37(2):429–438. doi: 10.1016/0003-2697(70)90069-2. [DOI] [PubMed] [Google Scholar]
  14. Ritenour G. L., Joy K. W., Bunning J., Hageman R. H. Intracellular localization of nitrate reductase, nitrite reductase, and glutamic Acid dehydrogenase in green leaf tissue. Plant Physiol. 1967 Feb;42(2):233–237. doi: 10.1104/pp.42.2.233. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Robinson J. M., Stocking C. R. Oxygen evolution and the permeability of the outer envelope of isolated whole chloroplasts. Plant Physiol. 1968 Oct;43(10):1597–1604. doi: 10.1104/pp.43.10.1597. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Santarius K. A., Stocking C. R. Intracellular localization of enzymes in leaves and chloroplast membrane permeability to compounds involved in amino acid syntheses. Z Naturforsch B. 1969 Sep;24(9):1170–1179. doi: 10.1515/znb-1969-0915. [DOI] [PubMed] [Google Scholar]
  17. Stepka W., Benson A. A., Calvin M. The Path of Carbon in Photosynthesis: II. Amino Acids. Science. 1948 Sep 17;108(2803):304–304. doi: 10.1126/science.108.2803.304. [DOI] [PubMed] [Google Scholar]
  18. Stocking C. R., Larson S. A chloroplast cytoplasmic shuttle and the reduction of extraplastid NAD. Biochem Biophys Res Commun. 1969 Oct 8;37(2):278–282. doi: 10.1016/0006-291x(69)90731-1. [DOI] [PubMed] [Google Scholar]
  19. Swader J. A., Stocking C. R. Nitrate and Nitrite Reduction by Wolffia arrhiza. Plant Physiol. 1971 Feb;47(2):189–191. doi: 10.1104/pp.47.2.189. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Tanner W., Loffler M., Kandler O. Cyclic Photophosphorylation in Vivo and its Relation to Photosynthetic CO(2)-Fixation. Plant Physiol. 1969 Mar;44(3):422–428. doi: 10.1104/pp.44.3.422. [DOI] [PMC free article] [PubMed] [Google Scholar]

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