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. 1975 Jan;55(1):94–98. doi: 10.1104/pp.55.1.94

Glycosidases in Cell Wall-degrading Extracts of Ripening Tomato Fruits

Stephen J Wallner a, John E Walker a
PMCID: PMC541558  PMID: 16659037

Abstract

Enzyme preparations were obtained from cell wall debris of tomato (Lycopersicon esculentum L. cv. Tropic) fruits at various stages of ripeness and were assayed for glycosidase and polysaccharidase activities. In addition to polygalacturonase (mol wt 40,000), ripening fruits contain β-galactosidase (mol wt 63,000) and β-1, 3-glucanase (mol wt 12,000). The β-glycosidases, unlike polygalacturonase, are active in extracts of green fruits. Placental tissue shows very low polygalacturonase but increasing β-galactosidase and β-1, 3-glucanase activities as ripening proceeds. A large change in the susceptibility of the walls to hydrolase action occurs before the stage in which the greatest polygalacturonase activity occurs. The possibility that the β-glycosidases contribute to the wall modifications that lead to fruit softening is discussed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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