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. 1975 Feb;55(2):212–217. doi: 10.1104/pp.55.2.212

Immunological and Physical Characterization of the Products of Phytochrome Proteolysis 1

Susan C Cundiff a, Lee H Pratt a
PMCID: PMC541586  PMID: 16659053

Abstract

The relationship between high molecular weight (large) and low molecular weight (small) forms of phytochrome has been shown earlier to be one of proteolysis. The products of such proteolysis are characterized here by chromatography through Bio-Gel P-200 using specific antiphytochrome sera as an assay system. Degradation of large oat (Avena sativa L. cv. Garry) phytochrome as phytochrome, red-absorbing form, phytochrome, far red-absorbing form, or under cycling conditions in crude preparations containing one or more proteases, always yields one fragment with the immunochemical, electrophoretic, spectroscopic, and size characteristics of small phytochrome. In addition, other fragments are detected which may account, in part, for the different molecular weight estimates reported by others for purified, photoreversible phytochrome. The small phytochrome produced by proteolysis with trypsin of a purified large phytochrome preparation is similar to that produced by the endogenously derived protease(s). A large (estimated molecular weight = 90,000), apparently nonphotoreversible peptide is also identified which is electrophoretically and immunochemically distinct from small phytochrome. Thus, it seems that small phytochrome may not represent more than approximately one-half of the native molecule.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BUTLER W. L., SIEGELMAN H. W., MILLER C. O. DENATURATION OF PHYTOCHROME. Biochemistry. 1964 Jun;3:851–857. doi: 10.1021/bi00894a022. [DOI] [PubMed] [Google Scholar]
  2. Butler W. L., Lane H. C., Siegelman H. W. Nonphotochemical Transformations of Phytochrome in Vivo. Plant Physiol. 1963 Sep;38(5):514–519. doi: 10.1104/pp.38.5.514. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Correll D. L., Edwards J. L. The aggregation States of phytochrome from etiolated rye and oat seedings. Plant Physiol. 1970 Jan;45(1):81–85. doi: 10.1104/pp.45.1.81. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Correll D. L., Steers E., Jr, Towe K. M., Shropshire W., Jr Phytochrome in etiolated annual rye. IV. Physical and chemical characterization of phytochrome. Biochim Biophys Acta. 1968 Sep 10;168(1):46–57. doi: 10.1016/0005-2795(68)90232-8. [DOI] [PubMed] [Google Scholar]
  5. Cundiff S. C., Pratt L. H. Immunological Determination of the Relationship between Large and Small Sizes of Phytochrome. Plant Physiol. 1973 Jan;51(1):210–213. doi: 10.1104/pp.51.1.210. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Cundiff S. C., Pratt L. H. Phytochrome Characterization by Rabbit Antiserum against High Molecular Weight Phytochrome. Plant Physiol. 1975 Feb;55(2):207–211. doi: 10.1104/pp.55.2.207. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Gardner G., Pike C. S., Rice H. V., Briggs W. R. "Disaggregation" of phytochrome in vitro-a consequence of proteolysis. Plant Physiol. 1971 Dec;48(6):686–693. doi: 10.1104/pp.48.6.686. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Hopkins D. W., Butler W. L. Immunochemical and spectroscopic evidence for protein conformational changes in phytochrome transformations. Plant Physiol. 1970 May;45(5):567–570. doi: 10.1104/pp.45.5.567. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Kidd G. H., Pratt L. H. Phytochrome destruction: an apparent requirement for protein synthesis in the induction of the destruction mechanism. Plant Physiol. 1973 Oct;52(4):309–311. doi: 10.1104/pp.52.4.309. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Mumford F. E., Jenner E. L. Purification and characterization of phytochrome from oat seedlings. Biochemistry. 1966 Nov;5(11):3657–3662. doi: 10.1021/bi00875a039. [DOI] [PubMed] [Google Scholar]
  11. Pike C. S., Briggs W. R. Partial Purification and Characterization of a Phytochrome-degrading Neutral Protease from Etiolated Oat Shoots. Plant Physiol. 1972 Apr;49(4):521–530. doi: 10.1104/pp.49.4.521. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Pratt L. H. Comparative immunochemistry of phytochrome. Plant Physiol. 1973 Jan;51(1):203–209. doi: 10.1104/pp.51.1.203. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Rice H. V., Briggs W. R. Immunochemistry of phytochrome. Plant Physiol. 1973 May;51(5):939–945. doi: 10.1104/pp.51.5.939. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Rice H. V., Briggs W. R. Partial characterization of oat and rye phytochrome. Plant Physiol. 1973 May;51(5):927–938. doi: 10.1104/pp.51.5.927. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Walker T. S., Bailey J. L. Studies on phytochrome. Some properties of electrophoretically pure phytochrome. Biochem J. 1970 Dec;120(3):613–622. doi: 10.1042/bj1200613. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Walker T. S., Bailey J. L. Studies on phytochrome. Two photoreversible chromoproteins from etiolated oat seedlings. Biochem J. 1970 Dec;120(3):607–612. doi: 10.1042/bj1200607. [DOI] [PMC free article] [PubMed] [Google Scholar]

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