Abstract
Apparent Km and Vmax values were obtained for hydrolysis of methyl and ethyl chlorophyllides a, methyl and ethyl pheophorbide a, and 9-hydroxymethyl pheophorbide a by chlorophyllase from Ailanthus altissima. Analysis of substrate specificity data for chlorophyllase indicates that the presence of a 9-keto group and a methyl alcohol group esterified at the 7-position in chlorophyll derivatives results in maximum binding affinity for substrates. Data on maximum reaction rates indicate that the rate-controlling step of hydrolysis occurs after release of the alcohol from the ester. Probable high affinity chlorophyllase inhibitors can be predicted on the basis of these specificity studies.
An improved method for purification of chlorophyllase has been developed.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Holt A. S. Reduction of Chlorophyllides, Chlorophylls and Chlorophyll Derivatives by Sodium Borohydride. Plant Physiol. 1959 May;34(3):310–314. doi: 10.1104/pp.34.3.310. [DOI] [PMC free article] [PubMed] [Google Scholar]
- KLEIN A. O., VISHNIAC W. Activity and partial purification of chlorophyllase in aqueous systems. J Biol Chem. 1961 Sep;236:2544–2547. [PubMed] [Google Scholar]
- LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
- McFeeters R. F., Chichester C. O., Whitaker J. R. Purification and Properties of Chlorophyllase from Ailanthus altissima (Tree-of-Heaven). Plant Physiol. 1971 May;47(5):609–618. doi: 10.1104/pp.47.5.609. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Schneider H. A. Eine einfache Methode zur dünnschichtchromatographischen Trennung von Plastidenpigmenten. J Chromatogr. 1966 Mar;21(3):448–453. doi: 10.1016/s0021-9673(01)91339-1. [DOI] [PubMed] [Google Scholar]
- WILKINSON G. N. Statistical estimations in enzyme kinetics. Biochem J. 1961 Aug;80:324–332. doi: 10.1042/bj0800324. [DOI] [PMC free article] [PubMed] [Google Scholar]