Abstract
Irradiating the mycelium of Neurospora crassa with moderate intensities of blue light causes a reversible photoreduction of a b-type cytochrome. The action spectrum for the photoreduction of cytochrome b is very similar to the absorption spectrum of flavin pigments. Prolonged irradiation of the mycelium with strong blue light irreversibly bleaches flavin-like pigments and as these pigments are bleached the photoresponse of cytochrome b is lost. We conclude from these and other data that a flavin is the photoreceptor pigment for the photoreduction of cytochrome b. The close similarity between the action spectrum for the photoreduction of cytochrome b and action spectra for a number of physiological photoresponses suggests that this photoreceptor pigment controls a wide variety of photobiological processes in a wide diversity of organisms.
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Selected References
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- Bruce V. G., Minis D. H. Circadian clock action spectrum in a photoperiodic moth. Science. 1969 Feb 7;163(3867):583–585. doi: 10.1126/science.163.3867.583. [DOI] [PubMed] [Google Scholar]
- Butler W. L. Absorption spectroscopy of biological materials. Methods Enzymol. 1972;24:3–25. doi: 10.1016/0076-6879(72)24052-6. [DOI] [PubMed] [Google Scholar]
- Chance B., Mayer D., Legallais V. A dual-wavelength spectrophotometer and fluorometer using interference filters. Anal Biochem. 1971 Aug;42(2):494–504. doi: 10.1016/0003-2697(71)90064-9. [DOI] [PubMed] [Google Scholar]
- Kowallik W. Action spectrum for an enhancement of endogenous respiration by light in chlorella. Plant Physiol. 1967 May;42(5):672–676. doi: 10.1104/pp.42.5.672. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Muñoz V., Brody S., Butler W. L. Photoreceptor pigment for blue light responses in Neurospora crassa. Biochem Biophys Res Commun. 1974 May 7;58(1):322–327. doi: 10.1016/0006-291x(74)90930-9. [DOI] [PubMed] [Google Scholar]
- Poff K. L., Butler W. L. Spectral characteristics of the photoreceptor pigment of phototaxis in Dictyostelium discoideum. Photochem Photobiol. 1974 Sep;20(3):241–244. doi: 10.1111/j.1751-1097.1974.tb06573.x. [DOI] [PubMed] [Google Scholar]
- RILLING H. C. ON THE MECHANISM OF PHOTOINDUCTION OF CAROTENOID SYNTHESIS. Biochim Biophys Acta. 1964 May 25;79:464–475. doi: 10.1016/0926-6577(64)90212-8. [DOI] [PubMed] [Google Scholar]
- Sargent M. L., Briggs W. R. The effects of light on a circadian rhythm of conidiation in neurospora. Plant Physiol. 1967 Nov;42(11):1504–1510. doi: 10.1104/pp.42.11.1504. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sargent M. L., Briggs W. R., Woodward D. O. Circadian nature of a rhythm expressed by an invertaseless strain of Neurospora crassa. Plant Physiol. 1966 Oct;41(8):1343–1349. doi: 10.1104/pp.41.8.1343. [DOI] [PMC free article] [PubMed] [Google Scholar]
- VERNON L. P. Photochemical oxidation and reduction reactions catalyzed by flavin nucleotides. Biochim Biophys Acta. 1959 Nov;36:177–185. doi: 10.1016/0006-3002(59)90082-4. [DOI] [PubMed] [Google Scholar]