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. 1975 Feb;55(2):427–429. doi: 10.1104/pp.55.2.427

Spectral Characterization of the Photoreducible b-Type Cytochrome of Dictyostelium discoideum1

Kenneth L Poff a,2, Warren L Butler a
PMCID: PMC541628  PMID: 16659095

Abstract

Irradiation of a soluble extract from broken cells of Dictyostelium discoideum causes the photoreduction of a b-type cytochrome. The cytochrome b can be separated from cytochrome c, which is also present in the extract, by column chromatography on Brushite, but the cytochrome b is no longer sensitive to light after separation on the column. Low temperature spectroscopy shows that reduced form of the photoreducible cytochrome b has a Soret band at 423 nm and a split α band with maxima at 558 and 551 nm similar to the b-type cytochrome in complex II of beef heart mitochondria.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bergman K., Burke P. V., Cerdá-Olmedo E., David C. N., Delbrück M., Foster K. W., Goodell E. W., Heisenberg M., Meissner G., Zalokar M. Phycomyces. Bacteriol Rev. 1969 Mar;33(1):99–157. doi: 10.1128/br.33.1.99-157.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Butler W. L. Absorption spectroscopy of biological materials. Methods Enzymol. 1972;24:3–25. doi: 10.1016/0076-6879(72)24052-6. [DOI] [PubMed] [Google Scholar]
  3. Davis K. A., Hatefi Y., Poff K. L., Butler W. L. The b-type cytochromes of bovine heart mitochondria: absorption spectra, enzymatic properties, and distribution in the electron transfer complexes. Biochim Biophys Acta. 1973 Dec 14;325(3):341–356. doi: 10.1016/0005-2728(73)90196-5. [DOI] [PubMed] [Google Scholar]
  4. Muñoz V., Brody S., Butler W. L. Photoreceptor pigment for blue light responses in Neurospora crassa. Biochem Biophys Res Commun. 1974 May 7;58(1):322–327. doi: 10.1016/0006-291x(74)90930-9. [DOI] [PubMed] [Google Scholar]
  5. Poff K. L., Butler W. L. Absorbance changes induced by blue light in Phycomyces blakesleeanus and Dictyostelium discoideum. Nature. 1974 Apr 26;248(5451):799–801. doi: 10.1038/248799a0. [DOI] [PubMed] [Google Scholar]
  6. Poff K. L., Loomis W. F., Jr, Butler W. L. Isolation and purification of the photoreceptor pigment associated with phototaxis in Dictyostelium discoideum. J Biol Chem. 1974 Apr 10;249(7):2164–2167. [PubMed] [Google Scholar]
  7. SIEGELMAN H. W., FIRER E. M. PURIFICATION OF PHYTOCHROME FROM OAT SEEDLINGS. Biochemistry. 1964 Mar;3:418–423. doi: 10.1021/bi00891a019. [DOI] [PubMed] [Google Scholar]
  8. Sargent M. L., Briggs W. R. The effects of light on a circadian rhythm of conidiation in neurospora. Plant Physiol. 1967 Nov;42(11):1504–1510. doi: 10.1104/pp.42.11.1504. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. ZALOKAR M. Biosynthesis of carotenoids in Neurospora; action spectrum of photoactivation. Arch Biochem Biophys. 1955 Jun;56(2):318–325. doi: 10.1016/0003-9861(55)90252-6. [DOI] [PubMed] [Google Scholar]

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