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. 1975 Mar;55(3):475–479. doi: 10.1104/pp.55.3.475

Isotope Fractionation in Photosynthetic Bacteria during Carbon Dioxide Assimilation 1

William Wong a, William M Sackett a, C Roy Benedict b
PMCID: PMC541641  PMID: 16659105

Abstract

The δ PDB13C values have been determined for the cellular constituents and metabolic intermediates of autotrophically grown Chromatium vinosum. The isotopic composition of the HCO3- in the medium and the carbon isotopic composition of the bacterial cells change with the growth of the culture. The δ PDB13C value of the HCO3- in the media changes from an initial value of −6.6‰ to +8.1‰ after 10 days of bacterial growth and the δ PDB13C value of the bacterial cells change from −37.5‰ to −29.2‰ in the same period. The amount of carbon isotope fractionation during the synthesis of hexoses by the photoassimilation of CO2 has a range of −15.5‰ at time zero to −22.0‰ after 10 days. This range of fractionation compares to the range of carbon isotope fractionation for the synthesis of sugars from CO2 by ribulose 1,5-diphosphate carboxylase and the Calvin cycle.

The amount of carbon isotope fractionation during the synthesis of aspartic acid from CO2 is −24.9‰ at time zero and −15.0‰ after 10 days of bacterial growth. This amount of fractionation is in the range of carbon isotope fractionation for the synthesis of C4 amino acids by a double carboxylation through ribulose 1,5-diphosphate and phosphoenolpyruvate carboxylase.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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