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. 1990 Jun;87(12):4620–4624. doi: 10.1073/pnas.87.12.4620

The priA gene encoding the primosomal replicative n' protein of Escherichia coli.

E H Lee 1, H Masai 1, G C Allen Jr 1, A Kornberg 1
PMCID: PMC54168  PMID: 2162050

Abstract

The Escherichia coli gene encoding protein n' has been isolated and named priA for primosomal protein A. Protein n' is absolutely required for the conversion of single-stranded phi X174 DNA to the duplex replicative form in an in vitro-reconstituted system. The gene maps to 88.7 minutes on the chromosome adjacent to the cytR locus. Soluble protein extracts from cells harboring the priA gene on a multicopy plasmid contained 45-fold more n' replication activity than wild-type extracts. Enhanced overproduction of greater than 1000-fold was achieved by replacing the natural Shine-Dalgarno sequence with that of the phage T7 phi 10 gene and placing this priA under the control of the T7 phage promoter and RNA polymerase. The priA sequence reveals a 732-amino acid open reading frame and a nucleotide-binding consensus site consistent with the size and ATPase activity of the purified protein. The gene for protein n has been named priB and the putative gene for protein n", priC.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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