Table 1.
Gene Set Enrichment Analysis reveals stronger stimulation of known E2 regulated genes in polysomes
| Pathway | E2 polysome vs. vehicle polysome | E2 total vs. vehicle total | ||||
|---|---|---|---|---|---|---|
| NES | P value | NES | P value | |||
| ER binding (genes within 10 kb) | 2.06 | 0.00 | 1.61 | 0.00 | ||
| Stossi E2 UP signature | 2.35 | 0.000 | 1.74 | 0.000 | ||
| Frasor E2 UP signature | 3.04 | 0.000 | 2.00 | 0.000 | ||
| GenMapp | ||||||
| Ribosomal proteins | 2.87 | 0.000 | 0.68 | 0.975 | ||
| Proteasome | 2.07 | 0.000 | −0.80 | 0.754 | ||
| Oxidative Phosphorylation | 1.75 | 0.000 | 0.69 | 0.952 | ||
| Glycolysis | 1.65 | 0.023 | 0.51 | 1.000 | ||
| DNA replication reactome | −0.83 | 0.738 | 1.42 | 0.034 | ||
| G1 to S Cell Cycle Reactome | 0.87 | 0.723 | 1.20 | 0.168 | ||
| ATP Synthesis | 1.17 | 0.261 | 0.51 | 0.985 | ||
| RNA Transcription Reactome | 1.44 | 0.060 | 1.59 | 0.016 | ||
| Apoptosis | 1.30 | 0.109 | 1.34 | 0.078 | ||
| Biocarta | ||||||
| ERK Pathway | 1.80 | 0.003 | 1.25 | 0.163 | ||
| WNT Pathway | 1.55 | 0.044 | 1.51 | 0.037 | ||
| MAPK Pathway | 1.43 | 0.022 | 1.43 | 0.021 | ||
| P38MAPK Pathway | 1.17 | 0.209 | 1.82 | 0.004 | ||
| CREB Pathway | 0.99 | 0.462 | 1.58 | 0.025 | ||
Normalized Enrichment Scores, NES, higher than 1.4 suggests significant bias towards E2. Positive NES scores indicate bias towards E2 while negative values indicate vehicle bias. P values < 0.1 are significant. Bold indicates possible translational regulation with significant E2 stimulation in the polysome level only. Italics indicates transcriptome-level E2 stimulation but not a translational response in the polysome comparisons.