In the article “Estrogen Coordinates Translation and Transcription, Revealing a Role for NRSF in Human Breast Cancer Cellsö” (Mol Endocrinol 24:1120–1135, 2010; doi:10.1210/me.2009-0436) by Michael W. Bronson, Sara Hillenmeyer, Richard W. Park and Alexander S. Brodsky, the following errors occurred:
In Figure 1B, the line Polysome vs. Total should read Translation State/Transcriptome Levels in the Analysis column, not Transcriptome State/transcriptome level. The line Polysome vs. Monosome should read Translation State in the Analysis column, not Transcriptome State. The line E2 Polysome vs. Veh Polysome should read Translation State/Transcriptome Levels in the Analysis column, not Transcriptome State/Transcriptome Levels. The correct figure is below. The printer regrets the errors.
Fig. 1.
In Table 1, errors occurred in the last two lines, P38MAPK pathway and CREB pathway. The data to the right should be in italics, not bold. The correct table is below. The printer regrets the errors.
Table 1.
Gene Set Enrichment Analysis reveals stronger stimulation of known E2 regulated genes in polysomes
| Pathway | E2 polysome vs. vehicle polysome | E2 total vs. vehicle total | ||||
|---|---|---|---|---|---|---|
| NES | P value | NES | P value | |||
| ER binding (genes within 10 kb) | 2.06 | 0.00 | 1.61 | 0.00 | ||
| Stossi E2 UP signature | 2.35 | 0.000 | 1.74 | 0.000 | ||
| Frasor E2 UP signature | 3.04 | 0.000 | 2.00 | 0.000 | ||
| GenMapp | ||||||
| Ribosomal proteins | 2.87 | 0.000 | 0.68 | 0.975 | ||
| Proteasome | 2.07 | 0.000 | −0.80 | 0.754 | ||
| Oxidative Phosphorylation | 1.75 | 0.000 | 0.69 | 0.952 | ||
| Glycolysis | 1.65 | 0.023 | 0.51 | 1.000 | ||
| DNA replication reactome | −0.83 | 0.738 | 1.42 | 0.034 | ||
| G1 to S Cell Cycle Reactome | 0.87 | 0.723 | 1.20 | 0.168 | ||
| ATP Synthesis | 1.17 | 0.261 | 0.51 | 0.985 | ||
| RNA Transcription Reactome | 1.44 | 0.060 | 1.59 | 0.016 | ||
| Apoptosis | 1.30 | 0.109 | 1.34 | 0.078 | ||
| Biocarta | ||||||
| ERK Pathway | 1.80 | 0.003 | 1.25 | 0.163 | ||
| WNT Pathway | 1.55 | 0.044 | 1.51 | 0.037 | ||
| MAPK Pathway | 1.43 | 0.022 | 1.43 | 0.021 | ||
| P38MAPK Pathway | 1.17 | 0.209 | 1.82 | 0.004 | ||
| CREB Pathway | 0.99 | 0.462 | 1.58 | 0.025 | ||
Normalized Enrichment Scores, NES, higher than 1.4 suggests significant bias towards E2. Positive NES scores indicate bias towards E2 while negative values indicate vehicle bias. P values < 0.1 are significant. Bold indicates possible translational regulation with significant E2 stimulation in the polysome level only. Italics indicates transcriptome-level E2 stimulation but not a translational response in the polysome comparisons.
