Skip to main content
PMC home page
Plant Physiology logoLink to Plant Physiology
. 1975 Jun;55(6):975–977. doi: 10.1104/pp.55.6.975

In Vitro Synthesis of Ureidohomoserine by an Enzyme from Jack Bean (Canavalia ensiformis) Leaves

Thomas Denny O'Neal 1
PMCID: PMC541749  PMID: 16659229

Abstract

An enzyme was extensively purified from jack bean leaves (Canavalia ensiformis L.) which produced o-ureidohomoserine from l-canaline and carbamyl phosphate. The most highly purified preparations catalyzed both this reaction and citrulline synthesis from ornithine and carbamyl phosphate, and the ratio of the two activities remained nearly constant during purification. When hydrated jack bean seeds were the enzyme source, ornithine carbamyltransferase (EC 2.1.3.3) activity was high but synthesis of ureidohomoserine was barely detectable. Both ornithine carbamyltransferase and the ureidohomoserine synthesizing enzyme had similar Km values for carbamyl phosphate. The purification data suggest that one enzyme may catalyze both reactions in jack bean leaves.

Full text

PDF
975

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. DAVIS B. J. DISC ELECTROPHORESIS. II. METHOD AND APPLICATION TO HUMAN SERUM PROTEINS. Ann N Y Acad Sci. 1964 Dec 28;121:404–427. doi: 10.1111/j.1749-6632.1964.tb14213.x. [DOI] [PubMed] [Google Scholar]
  2. Damodaran M., Narayanan K. G. A comparative study of arginase and canavanase. Biochem J. 1940 Nov;34(10-11):1449–1459. doi: 10.1042/bj0341449. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Hunninghake D., Grisolia S. A sensitive and convenient micromethod for estimation of urea, citrulline, and carbamyl derivatives. Anal Biochem. 1966 Aug;16(2):200–205. doi: 10.1016/0003-2697(66)90147-3. [DOI] [PubMed] [Google Scholar]
  4. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  5. Rosenthal G. A. Preparation and colorimetric analysis of O-ureido-L-homoserine. Anal Biochem. 1973 Dec;56(2):435–439. doi: 10.1016/0003-2697(73)90209-1. [DOI] [PubMed] [Google Scholar]
  6. Rosenthal G. A. The preparation and colorimetric analysis of L-canaline. Anal Biochem. 1973 Feb;51(2):354–361. doi: 10.1016/0003-2697(73)90488-0. [DOI] [PubMed] [Google Scholar]
  7. WALKER J. B. An enzymatic reaction between canavanine and fumarate. J Biol Chem. 1953 Sep;204(1):139–146. [PubMed] [Google Scholar]

Articles from Plant Physiology are provided here courtesy of Oxford University Press

RESOURCES