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. 1975 Jun;55(6):1115–1119. doi: 10.1104/pp.55.6.1115

Solubilization of Enzymes from Glyoxysomes of Maize Scutellum

Giovanna P Longo 1, Ermanno Bernasconi 1, Claudio P Longo 1
PMCID: PMC541778  PMID: 16659222

Abstract

Glyoxysomes isolated from maize scutella (Zea mays L.) were subjected to several disruptive treatments (osmotic shock, resuspension in an alkaline medium, addition of detergent). The damaged glyoxysomes were centrifuged at 89,500g for 40 minutes and several enzymic activities (isocitratase, malate synthetase, catalase, citrate synthetase, malate dehydrogenase) were measured in the supernatant fraction and in the pellet. Isocitratase is the most easily released of all glyoxysomal enzymes closely followed by malate synthetase. Citrate synthetase is in all instances the most insoluble enzyme. All of the enzymes had higher specific activities in the supernatant than in the pellet. These findings suggest that in corn scutellum glyoxysomes none of these enzymes is truly membrane-bound.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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