Abstract
A polysaccharide from the fungal pathogen Colletotrichum lindemuthianum causes browning and phytoalexin production when applied to the cut surfaces of bean (Phaseolus vulgaris) cotyledons and hypocotyls. The application of an amount of polysaccharide equivalent to less than 100 ng of glucose will elicit this response in the bean tissues. The polysaccharide has been isolated both from culture filtrates and from the mycelial walls of the fungus. Purification of the polysaccharide involved anion and cation exchange chromatography and gel filtration. The polysaccharide has an apparent molecular weight between 1,000,000 and 5,000,000 daltons, and consists predominantly of 3- and 4-linked glucosyl residues.
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Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Abeles F. B., Bosshart R. P., Forrence L. E., Habig W. H. Preparation and purification of glucanase and chitinase from bean leaves. Plant Physiol. 1971 Jan;47(1):129–134. doi: 10.1104/pp.47.1.129. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Albersheim P., Valent B. S. Host-Pathogen Interactions: VII. Plant Pathogens Secrete Proteins which Inhibit Enzymes of the Host Capable of Attacking the Pathogen. Plant Physiol. 1974 May;53(5):684–687. doi: 10.1104/pp.53.5.684. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ballou C. E. Some aspects of the structure, immunochemistry, and genetic control of yeast mannans. Adv Enzymol Relat Areas Mol Biol. 1974;40(0):239–270. doi: 10.1002/9780470122853.ch6. [DOI] [PubMed] [Google Scholar]
- Bauer W. D., Talmadge K. W., Keegstra K., Albersheim P. The Structure of Plant Cell Walls: II. The Hemicellulose of the Walls of Suspension-cultured Sycamore Cells. Plant Physiol. 1973 Jan;51(1):174–187. doi: 10.1104/pp.51.1.174. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Burlington R. F., Maher J. T., Angelakos E. T. Effect of temperature on contractility of isolated atria from a hibernator and a non-hibernator. Life Sci. 1968 May 1;7(9):449–452. doi: 10.1016/0024-3205(68)90046-5. [DOI] [PubMed] [Google Scholar]
- English P. D., Albersheim P. Host-Pathogen Interactions: I. A Correlation Between alpha-Galactosidase Production and Virulence. Plant Physiol. 1969 Feb;44(2):217–224. doi: 10.1104/pp.44.2.217. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Goodman R. N., Huang J. S., Huang P. Y. Host-Specific Phytotoxic Polysaccharide from Apple Tissue Infected by Erwinia amylovora. Science. 1974 Mar 15;183(4129):1081–1082. doi: 10.1126/science.183.4129.1081. [DOI] [PubMed] [Google Scholar]
- Hadwiger L. A., Jafri A., von Broembsen S., Eddy R. Mode of Pisatin Induction: Increased Template Activity and Dye-binding Capacity of Chromatin Isolated from Polypeptide-treated Pea Pods. Plant Physiol. 1974 Jan;53(1):52–63. doi: 10.1104/pp.53.1.52. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hadwiger L. A., Schwochau M. E. Induction of phenylalanine ammonia lyase and pisatin in pea pods by poly-lysine, spermidine or histone fractions. Biochem Biophys Res Commun. 1970 Feb 20;38(4):683–691. doi: 10.1016/0006-291x(70)90635-2. [DOI] [PubMed] [Google Scholar]
- Hadwiger L. A., Schwochau M. E. Specificity of deoxyribonucleic Acid intercalating compounds in the control of phenylalanine ammonia lyase and pisatin levels. Plant Physiol. 1971 Mar;47(3):346–351. doi: 10.1104/pp.47.3.346. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hadwiger L. A., Schwochau M. E. Ultraviolet Light-induced Formation of Pisatin and Phenylalanine Ammonia Lyase. Plant Physiol. 1971 Apr;47(4):588–590. doi: 10.1104/pp.47.4.588. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hess S. L., Hadwiger L. A. The induction of phenylalanine ammonia lyase and phaseollin by 9-aminoacridine and other deoxyribonucleic Acid intercalating compounds. Plant Physiol. 1971 Aug;48(2):197–202. doi: 10.1104/pp.48.2.197. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Jones T. M., Albersheim P. A gas chromatographic method for the determination of aldose and uronic Acid constituents of plant cell wall polysaccharides. Plant Physiol. 1972 Jun;49(6):926–936. doi: 10.1104/pp.49.6.926. [DOI] [PMC free article] [PubMed] [Google Scholar]
- LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
- Schwochau M. E., Hadwiger L. A. Regulation of gene expression by actinomycin D and other compounds which change the conformation of DNA. Arch Biochem Biophys. 1969 Oct;134(1):34–41. doi: 10.1016/0003-9861(69)90247-1. [DOI] [PubMed] [Google Scholar]
- Schwochau M. E., Hadwiger L. A. Stimulation of pisatin production in Pisum sativum by actinomycin D and other compounds. Arch Biochem Biophys. 1968 Aug;126(2):731–733. doi: 10.1016/0003-9861(68)90463-3. [DOI] [PubMed] [Google Scholar]
- Talmadge K. W., Keegstra K., Bauer W. D., Albersheim P. The Structure of Plant Cell Walls: I. The Macromolecular Components of the Walls of Suspension-cultured Sycamore Cells with a Detailed Analysis of the Pectic Polysaccharides. Plant Physiol. 1973 Jan;51(1):158–173. doi: 10.1104/pp.51.1.158. [DOI] [PMC free article] [PubMed] [Google Scholar]