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. 1975 Sep;56(3):410–414. doi: 10.1104/pp.56.3.410

A Simple, Inexpensive, High Pressure Liquid Chromatographic Method for Separating Cytokinins in Plant Extracts

Tudor H Thomas a,1, James E Carroll a, Francis M R Isenberg a, Ann Pendergrass a, Lydia Howell a
PMCID: PMC541834  PMID: 16659314

Abstract

Separation of a mixture of the main cytokinins occurring naturally in plant tissues was achieved by high pressure liquid chromatography using insoluble polyvinylpyrrolidone as the solid support. The separation of each cytokinin was first assessed over a range of salt and l-butanol concentrations and pH using a mixture of borate buffer and l-butanol as the mobile phase to determine the conditions necessary for optimum resolution. A discrete separation of zeatin, N-6-(Δ-2-isopentenyl)adenine, their related ribonucleosides, and kinetin was achieved using a simple isocratic elution with 0.025 m borate buffer at pH 6.8 and 4% (v/v) l-butanol. A number of cytokinin-active compounds were detected in cabbage extracts by the Amaranthus betacyanin bioassay using this separation technique.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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