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. 1975 Oct;56(4):481–485. doi: 10.1104/pp.56.4.481

Site of Action of Inhibitors of Carbon Dioxide Assimilation by Whole Lettuce Chloroplasts

Elchanan S Bamberger a,1, Mordhay Avron a
PMCID: PMC541854  PMID: 16659330

Abstract

The sites of action of several compounds, reported to inhibit CO2 fixation by chloroplast preparations were located by developing assays in lettuce chloroplasts to test their effect on partial reactions of the carbon cycle and on carbonic anhydrase. The results indicated that: d, l-glyceral-dehyde and 5′-AMP inhibit phosphoribulose kinase or isomerase. 3-Phosphoglyceric acid and 6-phosphogluconate inhibit ribulose diphosphate carboxylase. Azide, Mg2+, and nitrite inhibit the activity of carbonic anhydrase of lettuce chloroplasts and light-dependent CO2 fixation by intact chloroplasts with similar sensitivities. None of these inhibited CO2 fixation in ruptured chloroplasts. It is suggested that the inhibition by azide, nitrite, and magnesium ions of CO2 fixation by intact chloroplasts is due to their inhibition of the activity of carbonic anhydrase.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Andersen W. R., Gibbs M. Inhibition of CO2 fixation in intact spinach chloroplasts by 3-phosphoglyceric acid. Biochem Biophys Res Commun. 1975 Feb 17;62(4):953–956. doi: 10.1016/0006-291x(75)90415-5. [DOI] [PubMed] [Google Scholar]
  2. Anderson L. E. Chloroplast and cytoplasmic enzymes. 3. Pea leaf ribose 5-phosphate isomerases. Biochim Biophys Acta. 1971 Apr 14;235(1):245–249. doi: 10.1016/0005-2744(71)90052-0. [DOI] [PubMed] [Google Scholar]
  3. Avron M., Gibbs M. Carbon dioxide fixation in the light and in the dark by isolated spinach chloroplasts. Plant Physiol. 1974 Feb;53(2):140–143. doi: 10.1104/pp.53.2.140. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Avron M., Gibbs M. Properties of phosphoribulokinase of whole chloroplasts. Plant Physiol. 1974 Feb;53(2):136–139. doi: 10.1104/pp.53.2.136. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Bowes G. W. Carbonic anhydrase in marine algae. Plant Physiol. 1969 May;44(5):726–732. doi: 10.1104/pp.44.5.726. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Chu D. K., Bassham J. A. Inhibition of ribulose 1,5-diphosphate carboxylase by 6-phosphogluconate. Plant Physiol. 1972 Aug;50(2):224–227. doi: 10.1104/pp.50.2.224. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Forsee W. T., Kahn J. S. Carbon dioxide fixation by isolated chloroplasts of Egulena gracilis. II. Inhibition of CO 2 fixation by AMP. Arch Biochem Biophys. 1972 May;150(1):302–309. doi: 10.1016/0003-9861(72)90039-2. [DOI] [PubMed] [Google Scholar]
  8. Graham D., Reed M. L. Carbonic anhydrase and the regulation of photosynthesis. Nat New Biol. 1971 May 19;231(20):81–83. doi: 10.1038/newbio231081a0. [DOI] [PubMed] [Google Scholar]
  9. Jensen R. G. Activation of CO 2 fixation in isolated spinach chloroplasts. Biochim Biophys Acta. 1971 Jun 15;234(3):360–370. doi: 10.1016/0005-2728(71)90203-9. [DOI] [PubMed] [Google Scholar]
  10. Jensen R. G., Bassham J. A. Photosynthesis by isolated chloroplasts. II. Effects of addition of cofactors and intermediate compounds. Biochim Biophys Acta. 1968 Jan 15;153(1):219–226. doi: 10.1016/0005-2728(68)90163-1. [DOI] [PubMed] [Google Scholar]
  11. Poincelot R. P. Intracellular distribution of carbonic anhydrase in spinach leaves. Biochim Biophys Acta. 1972 Feb 28;258(2):637–642. doi: 10.1016/0005-2744(72)90255-0. [DOI] [PubMed] [Google Scholar]
  12. Stokes D. M., Walker D. A. Photosynthesis by isolated chloroplasts. Inhibition by DL-glyceraldehyde of carbon dioxide assimilation. Biochem J. 1972 Aug;128(5):1147–1157. doi: 10.1042/bj1281147. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Tabita F. R., McFadden B. A. Regulation of ribulose-1,5-diphosphate carboxylase by 6-phospho-D-gluconate. Biochem Biophys Res Commun. 1972 Sep 5;48(5):1153–1159. doi: 10.1016/0006-291x(72)90831-5. [DOI] [PubMed] [Google Scholar]
  14. Werdan K., Heldt H. W. Accumulation of bicarbonate in intact chloroplasts following a pH gradient. Biochim Biophys Acta. 1972 Dec 14;283(3):430–441. doi: 10.1016/0005-2728(72)90260-5. [DOI] [PubMed] [Google Scholar]

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