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. 1975 Nov;56(5):665–670. doi: 10.1104/pp.56.5.665

Studies with Detached Lupin Root Nodules in Culture

I. Maintenance and Induction of Acetylene Reduction Activity

William D Sutton 1, Neil M Jepsen 1
PMCID: PMC541894  PMID: 16659367

Abstract

A method has been developed for culturing detached nitrogen-fixing root nodules of lupin (Lupinus angustifolius L.) on a simple nutrient medium. Under the best conditions devised, the acetylene reduction activity of mature detached nodules was maintained at 10 to 25 nmoles of ethylene hr−1 mg−1 fresh weight for 3 days. Under the same culture conditions, immature nodules increased their acetylene reduction activity from 0.01 nmole or less to about 1 nmole hr−1 mg−1 fresh weight.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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