Skip to main content
PMC home page
Plant Physiology logoLink to Plant Physiology
. 1975 Nov;56(5):703–706. doi: 10.1104/pp.56.5.703

Effect of Temperature on Respiration of Mitochondria and Shoot Segments from Cold hardened and Nonhardened Wheat and Rye Seedlings 1

M Keith Pomeroy a, Chris J Andrews a
PMCID: PMC541903  PMID: 16659376

Abstract

The effect of temperature on respiration of mitochondria and tissue segments from three wheat (Triticum aestivum L.) and one rye (Secale cereale L.) cultivar grown at 2 and 24 C has been examined. Discontinuities in Arrhenius plots of respiratory activity against temperature were observed for mitochondria and tissue segments from seedlings grown at both temperatures. The rates of respiration decreased abruptly below the transition temperatures, resulting in increased energy of activation values for respiration. Transition temperatures were observed from 6 to 10 C during tissue segment respiration, and from 10 to 14 C during respiration by isolated mitochondria. Respiratory control and efficiency of phosphorylation were not affected markedly by either reaction temperature or growth temperature of the seedlings. No correlation was observed between the cold hardiness of the cultivars and the temperature at which structural transitions occurred in the mitochondria. Dry matter content of the seedlings increased markedly during growth at 2 C, but no appreciable changes in the levels of mitochondrial protein were observed. The results support the view that changes other than fatty acid unsaturation are involved in the abrupt change in mitochondrial membrane properties at low temperature.

Full text

PDF
703

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Breidenbach R. W., Wade N. L., Lyons J. M. Effect of chilling temperatures on the activities of glyoxysomal and mitochondrial enzymes from castor bean seedlings. Plant Physiol. 1974 Sep;54(3):324–327. doi: 10.1104/pp.54.3.324. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  3. Lyons J. M. Phase transitions and control of cellular metabolism at low temperatures. Cryobiology. 1972 Oct;9(5):341–350. doi: 10.1016/0011-2240(72)90152-6. [DOI] [PubMed] [Google Scholar]
  4. Lyons J. M., Raison J. K. Oxidative activity of mitochondria isolated from plant tissues sensitive and resistant to chilling injury. Plant Physiol. 1970 Apr;45(4):386–389. doi: 10.1104/pp.45.4.386. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Lyons J. M., Wheaton T. A., Pratt H. K. Relationship between the Physical Nature of Mitochondrial Membranes and Chilling Sensitivity in Plants. Plant Physiol. 1964 Mar;39(2):262–268. doi: 10.1104/pp.39.2.262. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. McGlasson W. B., Raison J. K. Occurrence of a Temperature-induced Phase Transition in Mitochondria Isolated from Apple Fruit. Plant Physiol. 1973 Oct;52(4):390–392. doi: 10.1104/pp.52.4.390. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Miller R. W., de la Roche I., Pomeroy M. K. Structural and functional responses of wheat mitochondrial membranes to growth at low temperatures. Plant Physiol. 1974 Mar;53(3):426–433. doi: 10.1104/pp.53.3.426. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Pomeroy M. K. Studies on the respiratory properties of mitochondria isolated from developing winter wheat seedlings. Plant Physiol. 1974 Apr;53(4):653–657. doi: 10.1104/pp.53.4.653. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Raison J. K., Lyons J. M., Mehlhorn R. J., Keith A. D. Temperature-induced phase changes in mitochondrial membranes detected by spin labeling. J Biol Chem. 1971 Jun 25;246(12):4036–4040. [PubMed] [Google Scholar]
  10. Raison J. K., Lyons J. M., Thomson W. W. The influence of membranes on the temperature-induced changes in the kinetics of some respiratory enzymes of mitochondria. Arch Biochem Biophys. 1971 Jan;142(1):83–90. doi: 10.1016/0003-9861(71)90261-x. [DOI] [PubMed] [Google Scholar]
  11. Raison J. K., McMurchie E. J. Two temperature-induced changes in mitochondrial membranes detected by spin labelling and enzyme kinetics. Biochim Biophys Acta. 1974 Sep 6;363(2):135–140. doi: 10.1016/0005-2736(74)90053-4. [DOI] [PubMed] [Google Scholar]
  12. Wade N. L., Breidenbach R. W., Lyons J. M. Temperature-induced Phase Changes in the Membranes of Glyoxysomes, Mitochondria, and Proplastids from Germinating Castor Bean Endosperm. Plant Physiol. 1974 Sep;54(3):320–323. doi: 10.1104/pp.54.3.320. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Plant Physiology are provided here courtesy of Oxford University Press

RESOURCES