Table 1.
N-glycan Profiles from Arabinose Supplemented Cultures.
| D-Arabinose Conc. (mM) | Man5 (%) | Man6 (%) | G0A- GlcNAc (%) | G0F- GlcNAc (%) | G0(%) | G0A(%) | G0F (%) | G1A (%) | G1F (%) | Fucose Reduction (%) |
|---|---|---|---|---|---|---|---|---|---|---|
| Cell Line 1 (n = 3) | ||||||||||
| 0 | 2.1+/−0.1 | 1.0+/−0.1 | 0 | 8.6+/−0.1 | 0 | 0 | 83.3+/−0.6 | 0 | 3.6+/−0.1 | 3.2+/−0.1 |
| 0.1 | 1.9+/−0.1 | 1.0+/−0.1 | 0 | 8.5+/−0.1 | 0 | 0 | 82.6+/−0.1 | 0 | 3.4+/−0.0 | 2.8+/−0.1 |
| 1 | 2.7+/−0.1 | 0.3+/−0.5 | 0 | 7.1+/−0.1 | 0.5+/−0.1 | 13.6+/−1.2 | 70.1+/−0.9 | 0.7+/−0.1 | 3.1+/−0.1 | 17.9 +/− 1.0 |
| 10 | 0 | 1.0+/−0.0 | 10.8+/−0.3 | 0 | 3.5+/−0.1 | 79.6+/−0.5 | 0 | 3.2+/−0.1 | 0 | 98.2+/−0.2 |
| 20 | 0 | 1.5+/−0.1 | 13.4+/−0.4 | 0 | 3.5+/−0.1 | 76.3+/−0.4 | 0 | 3.4+/−0.1 | 0 | 98.1+/−0.1 |
| 50 | 0 | 3.6+/−0.1 | 11.7+/−0.3 | 0 | 2.7+/−0.0 | 73.7+/−0.5 | 0 | 6.4+/−0.0 | 0 | 98.1+/−0.2 |
| Cell Line 2 (n = 3) | ||||||||||
| 0 | 2.1+/−0.0 | 0 | 0 | 6.6+/−0.2 | 0 | 0 | 85.0+/−0.3 | 0 | 4.4+/−0.2 | 2.1+/−0.0 |
| 0.1 | 2.1+/−0.0 | 0 | 0 | 6.5+/−0.0 | 0 | 0 | 85.3+/−0.1 | 0 | 4.2+/−0.1 | 2.1+/−0.0 |
| 1 | 2.4+/−0.1 | 0 | 0 | 5.9+/−0.1 | 0 | 0 | 85.1+/−0.3 | 0 | 4.8+/−0.2 | 2.4+/−0.1 |
| 10 | 2.1+/−0.1 | 0.5+/−0.4 | 2.8+/−0.0 | 3.6+/−0.0 | 3.8+/−0.0 | 34.5+/−0.4 | 48.3+/−0.3 | 1.2+/−0.0 | 2.6+/−0.2 | 44.9+/−0.6 |
| 20 | 2.5+/−0.0 | 2.1+/−0.2 | 3.5+/−0.0 | 3.1+/−0.0 | 4.2+/−0.1 | 38.8+/−0.2 | 40.9+/−0.2 | 1.6+/−0.0 | 2.4+/−0.1 | 52.7+/−0.3 |
| 50 | 5.3+/−0.3 | 5.0+/−0.1 | 3.8+/−0.2 | 4.0+/−0.1 | 4.4+/−0.0 | 33.5+/−0.3 | 37.8+/−0.3 | 2.1+/−0.0 | 3.3+/−0.0 | 54.1+/−0.1 |
| Cell Line 3 (n = 3) | ||||||||||
| 0 | 1.8+/−0.1 | 0 | 0 | 6.6+/−0.1 | 0.2+/−0.1 | 0 | 85.0+/−0.6 | 0 | 4.2+/−0.4 | 1.9+/−0.1 |
| 0.1 | 1.6+/−0.1 | 0 | 0 | 6.2+/−0.2 | 0.2+/−0.3 | 0 | 86.7+/−0.9 | 0 | 3.6+/−0.1 | 1.6+/−0.2 |
| 1 | 0 | 0 | 2.8+/−0.5 | 3.6+/−0.0 | 1.2+/−0.0 | 34.2+/−0.2 | 53.7+/−0.6 | 0.6+/−0.0 | 3.2+/−0.1 | 38.8+/−0.7 |
| 10 | 0 | 0 | 7.5+/−0.2 | 0 | 2.8+/−0.0 | 84.8+/−0.6 | 0 | 2.9+/−0.4 | 0 | 98.0+/−0.1 |
| 20 | 0 | 0 | 7.8+/−0.2 | 0 | 2.9+/−0.0 | 84.0+/−0.2 | 0 | 3.3+/−0.2 | 0 | 98.0+/−0.1 |
| 50 | 0 | 0 | 10.4+/−0.3 | 0 | 2.8+/−0.0 | 81.0+/−0.5 | 0 | 3.6+/−0.5 | 0 | 98.7+/−0.0 |
Note: Glycan %'s represent mean +/− 1 SD.