Figure 10. Double reciprocal plot of 1/[S] (L/mmol) and 1/v (10⁴ Ls/mmol).

PL and pNPB were mixed and incubated in the water bath at 37°C for exactly 15 min. Then, immediately, the contents were cooled for 10 min on ice to stop the reaction and centrifuged at 3,740 × g and in the range of 2–4°C for 5 min. The supernatants were pipetted to measure the absorbance of the released pNP spectrophotometrically at 405 nm against the reagent blank. Data are expressed as the means ± SD from triplicate experiments. Different small letters next to values indicate significant differences (p < 0.05).