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. 1975 Dec;56(6):850–852. doi: 10.1104/pp.56.6.850

Isolation of Nucleoli and Localization of Ribonucleic Acid Polymerase I from Soybean Hypocotyl 1

Chu-Yung Lin a,2, Thomas J Guilfoyle a, Yih-Ming Chen a,2, Joe L Key a
PMCID: PMC541939  PMID: 16659409

Abstract

An effective method for the isolation of nucleoli from auxin-treated soybean (Glycine max, var. Wayne) hypocotyl was developed by polytron homogenization and sucrose gradient centrifugation. The nucleoli expressed only the α-amanitin-insensitive RNA synthetic activity. This activity chromatographed as RNA polymerase I on DEAE-cellulose. It appears that the plant nucleolus, like the animal nucleolus, is the site of localization for RNA polymerase I.

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Selected References

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