Abstract
Assimilatory nitrate reductase (NADH: nitrate oxidoreductase, EC 1.6.6.1) from Chlorella vulgaris has been purified to electrophoretic homogeneity with an overall yield of 60% by a procedure that utilizes blue dextran-agarose as an affinity column. Nitrate reductase binds to blue dextran and is not eluted in the presence of high ionic strength buffer but is rapidly eluted in the presence of μmolar concentrations of NADH.
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Selected References
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- Garrett R. H., Nason A. Further purification and properties of Neurospora nitrate reductase. J Biol Chem. 1969 Jun 10;244(11):2870–2882. [PubMed] [Google Scholar]
- Jetschmann K., Solomonson L. P., Vennesland B. Activation of nitrate reductase by oxidation. Biochim Biophys Acta. 1972 Aug 17;275(2):276–278. doi: 10.1016/0005-2728(72)90048-5. [DOI] [PubMed] [Google Scholar]
- Kopperschläger G., Diezel W., Freyer R., Liebe S., Hofmann E. Wechselwirkungen der Hefe-Phosphofructokinase mit Dextranblau 2000. Eur J Biochem. 1971 Sep 13;22(1):40–45. doi: 10.1111/j.1432-1033.1971.tb01512.x. [DOI] [PubMed] [Google Scholar]
- Ryan L. D., Vestling C. S. Rapid purification of lactate dehydrogenase from rat liver and hepatoma: a new approach. Arch Biochem Biophys. 1974 Jan;160(1):279–284. doi: 10.1016/s0003-9861(74)80035-4. [DOI] [PubMed] [Google Scholar]
- Solomonson L. P., Vennesland B. Properties of a nitrate reductase of Chlorella. Biochim Biophys Acta. 1972 Jun 23;267(3):544–557. doi: 10.1016/0005-2728(72)90183-1. [DOI] [PubMed] [Google Scholar]
- Thompson S. T., Cass K. H., Stellwagen E. Blue dextran-sepharose: an affinity column for the dinucleotide fold in proteins. Proc Natl Acad Sci U S A. 1975 Feb;72(2):669–672. doi: 10.1073/pnas.72.2.669. [DOI] [PMC free article] [PubMed] [Google Scholar]