Skip to main content
NCBI home page
Plant Physiology logoLink to Plant Physiology
. 1976 Feb;57(2):142–147. doi: 10.1104/pp.57.2.142

Separation and Characterization of Potato Lipid Acylhydrolases 1,2

Edna P Hasson a, George G Laties a
PMCID: PMC541979  PMID: 16659438

Abstract

Three distinct potato (Solanum tuberosum) lipid acyl-hydrolases have been isolated and characterized. Nonfluorescent esters of the fluorescent alcohols, N-methylindoxyl and N-methylumbelliferone, have been used as convenient substrates for lipid acyl-hydrolase estimation. Enzyme I has been shown to be a neutral lipase which favors glyceryl triolein over the di- and monoolein, which shows no activity with phospho- and galactolipids and which favors long chain fatty acid esters of N-methylindoxyl over the butyrate ester. Enzyme II, while attacking glyceryl mono- and diolein, as well as favoring the butyrate ester of N-methylindoxyl over the myristate ester, is basically a phospholipid and galactolipid acyl-hydrolase. Enzyme III may reasonably be considered an esterase, since it hydrolyzes glyceryl monoolein exclusively among the neutral lipids, shows minimal activity on phospho- and galactolipids, and hydrolyzes N-methylindoxylbutyrate exclusively compared with N-methylindoxyl-myristate.

Full text

PDF
142

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Anderson M. M., McCarty R. E. Rapid and sensitive assay for free fatty acids using rhodamine 6G. Anal Biochem. 1972 Jan;45(1):260–270. doi: 10.1016/0003-2697(72)90026-7. [DOI] [PubMed] [Google Scholar]
  2. Davídek J., Seifert J. Polarographic determination of esterase and lipase activities using esters of -naphthols. Anal Biochem. 1973 May;53(1):184–190. doi: 10.1016/0003-2697(73)90420-x. [DOI] [PubMed] [Google Scholar]
  3. Elliott W. B., Panagides K. Hydrolysis of N-methyl indoxyl acetate by a noncholine esterase type of esterase. Anal Biochem. 1972 Feb;45(2):345–348. doi: 10.1016/0003-2697(72)90197-2. [DOI] [PubMed] [Google Scholar]
  4. Fleisher M., Schwartz M. K. An automated, fluorometric procedure for determining serum lipase. Clin Chem. 1971 May;17(5):417–422. [PubMed] [Google Scholar]
  5. Galliard T. The enzymic deacylation of phospholipids and galactolipids in plants. Purification and properties of a lipolytic acyl-hydrolase from potato tubers. Biochem J. 1971 Feb;121(3):379–390. doi: 10.1042/bj1210379. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Hirayama O., Matsuda H., Takeda H., Maenaka K., Takatsuka H. Purification and properties of a lipid acyl-hydrolase from potato tubers. Biochim Biophys Acta. 1975 Mar 28;384(1):127–137. doi: 10.1016/0005-2744(75)90102-3. [DOI] [PubMed] [Google Scholar]
  7. Jacobson B. S., Smith B. N., Epstein S., Laties G. G. The prevalence of carbon-13 in respiratory carbon dioxide as an indicator of the types of endogenous substrate. The change from lipid to carbohydrate during the respiratory rise in potato slices. J Gen Physiol. 1970 Jan;55(1):1–17. doi: 10.1085/jgp.55.1.1. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Katocs A. S., Jr, Gnewuch C. T., Lech J. J., Calvert D. N. Further studies of monoolein lipase from rat adipose tissue. Biochim Biophys Acta. 1972 Jun 19;270(2):209–217. doi: 10.1016/0005-2760(72)90232-9. [DOI] [PubMed] [Google Scholar]
  9. Mattson F. H., Volpenhein R. A. Enzymatic hydrolysis at an oil-water interface. J Am Oil Chem Soc. 1966 May;43(5):286–289. doi: 10.1007/BF02609675. [DOI] [PubMed] [Google Scholar]
  10. Muto S., Beevers H. Lipase Activities in Castor Bean Endosperm during Germination. Plant Physiol. 1974 Jul;54(1):23–28. doi: 10.1104/pp.54.1.23. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Ory R. L., Yatsu L. Y., Kircher H. W. Association of lipase activity with the spherosomes of Ricinus communis. Arch Biochem Biophys. 1968 Feb;123(2):255–264. doi: 10.1016/0003-9861(68)90132-x. [DOI] [PubMed] [Google Scholar]
  12. SASTRY P. S., KATES M. HYDROLYSIS OF MONOGALACTOSYL AND DIGALACTOSYL DIGLYCERIDES BY SPECIFIC ENZYMES IN RUNNER-BEAN LEAVES. Biochemistry. 1964 Sep;3:1280–1287. doi: 10.1021/bi00897a016. [DOI] [PubMed] [Google Scholar]
  13. Schotz M. C., Garfinkel A. S. A simple lipase assay using trichloroacetic acid. J Lipid Res. 1972 Nov;13(6):824–826. [PubMed] [Google Scholar]
  14. Verger R., Sarda L., Desnuelle P. On the sulfhydryl groups of porcine pancreatic lipase and their possible role in the activity of the enzyme. Biochim Biophys Acta. 1971 Sep 22;242(3):580–592. doi: 10.1016/0005-2744(71)90151-3. [DOI] [PubMed] [Google Scholar]
  15. Wills E. D. Lipases. Adv Lipid Res. 1965;3:197–240. doi: 10.1016/b978-1-4831-9939-9.50012-7. [DOI] [PubMed] [Google Scholar]

Articles from Plant Physiology are provided here courtesy of Oxford University Press

RESOURCES