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. 1976 Feb;57(2):175–178. doi: 10.1104/pp.57.2.175

Response of Barley Aleurone Layers to Abscisic Acid 1

David Tuan-Hua Ho a, Joseph E Varner b
PMCID: PMC541986  PMID: 16659445

Abstract

Cordycepin, an inhibitor of RNA synthesis in barley (Hordeum vulgare L.) aleurone cells, does not inhibit the gibberellic acid-enhanced α-amylase (EC 3.2.1.1.) synthesis in barley aleurone layers if it is added 12 hours or more after the addition of the hormone. However, the accumulation of α-amylase activity after 12 hours of gibberellic acid can be decreased by abscisic acid. The accumulation of α-amylase activity is sustained or quickly restored when cordycepin is added simultaneously or some time after abscisic acid, indicating that the response of aleurone layers to abscisic acid depends on the continuous synthesis of a short lived RNA. By analysis of the newly synthesized proteins by gel electrophoresis with sodium dodecylsulfate, we observed that the synthesis of α-amylase is decreased in the presence of abscisic acid while the synthesis of most of the other proteins remains unchanged. From the rate of resumption of α-amylase production in the presence of cordycepin and abscisic acid, it appears that abscisic acid does not have a measurable effect on the stability of α-amylase mRNA.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ben-Tal Y. An early response to gibberellic Acid not requiring protein synthesis. Plant Physiol. 1974 Dec;54(6):813–816. doi: 10.1104/pp.54.6.813. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Chrispeels M. J., Varner J. E. Gibberellic Acid-enhanced synthesis and release of alpha-amylase and ribonuclease by isolated barley and aleurone layers. Plant Physiol. 1967 Mar;42(3):398–406. doi: 10.1104/pp.42.3.398. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Chrispeels M. J., Varner J. E. Hormonal control of enzyme synthesis: on the mode of action of gibberellic Acid and abscisin in aleurone layers of barley. Plant Physiol. 1967 Jul;42(7):1008–1016. doi: 10.1104/pp.42.7.1008. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Evins W. H., Varner J. E. Hormonal control of polyribosome formation in barley aleurone layers. Plant Physiol. 1972 Mar;49(3):348–352. doi: 10.1104/pp.49.3.348. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Evins W. H., Varner J. E. Hormone-controlled synthesis of endoplasmic reticulum in barley aleurone cells. Proc Natl Acad Sci U S A. 1971 Jul;68(7):1631–1633. doi: 10.1073/pnas.68.7.1631. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Fuhr J. E., Natta C. Translational control of and globin chain synthesis. Nat New Biol. 1972 Dec 27;240(104):274–276. doi: 10.1038/newbio240274a0. [DOI] [PubMed] [Google Scholar]
  7. Fuhr J. E., Overton M. Translational control of globin synthesis by low molecular weight RNA. Biochem Biophys Res Commun. 1975 Apr 7;63(3):742–747. doi: 10.1016/s0006-291x(75)80446-3. [DOI] [PubMed] [Google Scholar]
  8. Ho D. T., Varner J. E. Hormonal control of messenger ribonucleic acid metabolism in barley aleurone layers. Proc Natl Acad Sci U S A. 1974 Dec;71(12):4783–4786. doi: 10.1073/pnas.71.12.4783. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Ihle J. N., Dure L., 3rd Hormonal regulation of translation inhibition requiring RNA synthesis. Biochem Biophys Res Commun. 1970 Mar 27;38(6):995–1001. doi: 10.1016/0006-291x(70)90338-4. [DOI] [PubMed] [Google Scholar]
  10. Jacobsen J. V. Interactions between Gibberellic Acid, Ethylene, and Abscisic Acid in Control of Amylase Synthesis in Barley Aleurone Layers. Plant Physiol. 1973 Jan;51(1):198–202. doi: 10.1104/pp.51.1.198. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Jacobsen J. V., Zwar J. A. Gibberellic acid causes increased synthesis of RNA which contains poly(A) in barley aleurone tissue. Proc Natl Acad Sci U S A. 1974 Aug;71(8):3290–3293. doi: 10.1073/pnas.71.8.3290. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Johnson K. D., Kende H. Hormonal Control of Lecithin Synthesis in Barley Aleurone Cells: Regulation of the CDP-Choline Pathway by Gibberellin. Proc Natl Acad Sci U S A. 1971 Nov;68(11):2674–2677. doi: 10.1073/pnas.68.11.2674. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Koehler D. E., Varner J. E. Hormonal control of orthophosphate incorporation into phospholipids of barley aleurone layers. Plant Physiol. 1973 Sep;52(3):208–214. doi: 10.1104/pp.52.3.208. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  15. Mousseron-Canet M., Mani J. C., Durand B., Nitsch J., Dornand J., Bonnafous J. C. Analogues de l'acide abscisique (plus or minus) hormone de dormance. Relations structure-activité. C R Acad Sci Hebd Seances Acad Sci D. 1970 Apr 13;270(15):1936–1939. [PubMed] [Google Scholar]
  16. Varner J. E., Mense R. M. Characteristics of the process of enzyme release from secretory plant cells. Plant Physiol. 1972 Feb;49(2):187–189. doi: 10.1104/pp.49.2.187. [DOI] [PMC free article] [PubMed] [Google Scholar]

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