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. 1976 Mar;57(3):339–343. doi: 10.1104/pp.57.3.339

Glutamine Synthetase Regulation by Energy Charge in Sunflower Roots 1

Gerard S Weissman a
PMCID: PMC542021  PMID: 16659478

Abstract

Energy charge [(ATP) + ½ (ADP)]/[(ATP) + (ADP) + (AMP)] and glutamine synthetase activity (transferase reaction) of roots increase in a near congruent manner when decotyledonized sunflower plants (Helianthus annuus L. var. Mammoth Russian) are grown in nitrate for 9 days. Replacement of nitrate with ammonium for the final 2 days leads to a higher energy charge and increased enzyme activity. Similar correlations occur when nitrate plants are placed on a zero nitrogen regimen and when they are subjected to continuous darkness. A rank order correlation of 0.72 is obtained for all data. Control concepts such as adenylylation-deadenylylation and ammonium inhibition of enzyme synthesis are not supported by the data. Energy charge-enzyme activity plots support the view that glutamine synthetase of sunflower roots is subject to control by end products of glutamine metabolism. Alanine appears to exert a modulating effect on the regulation of glutamine synthetase by energy charge.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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