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. 1976 Mar;57(3):446–449. doi: 10.1104/pp.57.3.446

Promotive Effects of Organic Solvents and Kinetin on Dark Germination of Lettuce Seeds 1,2

V Sivaji Rao a,3, Joseph W Braun a, Anwar A Khan a
PMCID: PMC542043  PMID: 16659500

Abstract

Significant promotion in dark germination was observed when Grand Rapids lettuce (Lactuca sativa L.) seeds were soaked in acetone or dichloromethane, vacuum-dried, and imbibed at 25 C. Permeation of kinetin via these organic solvents further enhanced the dark germination. Those seeds that were affected by acetone and acetone-kinetin treatments and germinated in the dark escaped red-far red photocontrol of germination. Although abscisic acid was not detected in the organic solvent leachates, they did contain other inhibitory substances affecting lettuce seedling growth. In the light, acetone and acetone-kinetin treatments also enhanced the rate of germination and the increased germination by acetone-kinetin treatment was correlated with increased polyribosome formation. The possible mechanisms involved in promotion of lettuce seed germination by organic solvents and kinetin are discussed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Black M. Light-controlled germination of seeds. Symp Soc Exp Biol. 1969;23:193–217. [PubMed] [Google Scholar]
  2. Braun J. W., Khan A. A. Endogenous abscisic Acid levels in germinating and nongerminating lettuce seed. Plant Physiol. 1975 Dec;56(6):731–733. doi: 10.1104/pp.56.6.731. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. EVENARI M. The physiological action and biological importance of germination inhibitors. Symp Soc Exp Biol. 1957;11:21–44. [PubMed] [Google Scholar]
  4. Kahn A. Promotion of Lettuce Seed Germination by Gibberellin. Plant Physiol. 1960 May;35(3):333–339. doi: 10.1104/pp.35.3.333. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Khan A. A. Cytokinins: permissive role in seed germination. Science. 1971 Mar 5;171(3974):853–859. doi: 10.1126/science.171.3974.853. [DOI] [PubMed] [Google Scholar]
  6. Khan A. A. Inhibition of Gibberellic Acid-induced Germination by Abscisic Acid and Reversal by Cytokinins. Plant Physiol. 1968 Sep;43(9):1463–1465. doi: 10.1104/pp.43.9.1463. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Khan A. A., Tao K. L., Roe C. H. Application of chemicals in organic solvents to dry seeds. Plant Physiol. 1973 Jul;52(1):79–81. doi: 10.1104/pp.52.1.79. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Meyer H., Mayer A. M. Permeation of dry seeds with chemicals: use of dichloromethane. Science. 1971 Feb 12;171(3971):583–584. doi: 10.1126/science.171.3971.583. [DOI] [PubMed] [Google Scholar]
  9. Miller C. O. The Relationship of the Kinetin and Red-Light Promotions of Lettuce Seed Germination. Plant Physiol. 1958 Mar;33(2):115–117. doi: 10.1104/pp.33.2.115. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Park W. M., Chen S. S. Patterns of food utilization by the germinating lettuce seeds. Plant Physiol. 1974 Jan;53(1):64–66. doi: 10.1104/pp.53.1.64. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Rao V. S., Sankhla N., Khan A. A. Additive and synergistic effects of kinetin and ethrel on germination, thermodormany, and polyribosome formation in lettuce seeds. Plant Physiol. 1975 Aug;56(2):263–266. doi: 10.1104/pp.56.2.263. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Scheibe J., Lang A. Lettuce Seed Germination: Evidence for a Reversible Light-Induced Increase in Growth Potential and for Phytochrome Mediation of the Low Temperature Effect. Plant Physiol. 1965 May;40(3):485–492. doi: 10.1104/pp.40.3.485. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Skinner C. G., Shive W. Stimulation of Lettuce Seed Germination by 6-(Substituted) Purines. Plant Physiol. 1959 Jan;34(1):1–3. doi: 10.1104/pp.34.1.1. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Tao K. L., Khan A. A. Penetration of dry seeds with chemicals applied in acetone. Plant Physiol. 1974 Dec;54(6):956–958. doi: 10.1104/pp.54.6.956. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Thompson R. C., Kosar W. F. STIMULATION OF GERMINATION OF DORMANT LETTUCE SEED BY SULPHUR COMPOUNDS. Plant Physiol. 1939 Jul;14(3):567–573. doi: 10.1104/pp.14.3.567. [DOI] [PMC free article] [PubMed] [Google Scholar]

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