Table I.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Genotype and use or description | Source or reference |
|---|---|---|
| Escherichia coli strain | ||
| DH5α | F– Φ80lacZΔM15 Δ(lacZYA-argF) U169 recA1 endA1 hsdR17 (rK–, mK+) phoA supE44 λ– thi-1 gyrA96 relA1; cloning strain | Invitrogen, Austria |
| Tannerella forsythia strains | ||
| ATCC 43037 | Type strain, wild-type | ATCC; Friedrich et al. (2015) |
| ATCC 43037 ΔTanf_01245 | ΔTanf_01245::ermF; knock-out strain of Tanf_01245 | This work |
| ATCC 43037 ΔTanf_01245+ | ΔTanf_01245::Tanf_01245 cat; reconstituted knock-out strain | This work |
| ATCC 43037 ΔTanf_01245+TFUB4_00887 | ΔTanf_01245:: TFUB4_00887cat; cross-complemented knock-out strain | This work |
| ATCC 43037 ΔpseC | ΔpseC::ermF; knock-out strain of Tanf_01190 | Friedrich et al. (2017) |
| UB4 | Clinical isolate; wild-type strain | Stafford et al. (2016) |
| UB4 ΔTFUB4_00887 | ΔTFUB4_00887::ermF; knock-out strain of TFUB4_00887 | This work |
| UB4 ΔTFUB4_00887+ | ΔTFUB4_00887::cat; reconstituted knock-out strain | This work |
| UB4 ΔTFUB4_00887+Tanf_01245 | ΔTFUB4_00887::Tanf_01245 cat; cross-complemented knock-out strain | This work |
| UB4 ΔlegC | ΔlegC::ermF; knock-out strain of TFUB4_00900 | Friedrich et al. (2017) |
| FDC 92A2 | Wild-type strain | ATCC; Tanner et al. (1986) |
| Plasmids | ||
| pJET1.2/blunt | Cloning vector; ampR | Thermo Scientific, Austria |
| pJET/ΔTF0955ko | Vector for amplification of the erythromycin resistance gene | Tomek et al. (2014) |
| pEXALV | Vector for amplification of the Cat resistance gene | Zarschler et al. (2009) |
| pJET1.2/ΔTanf_01245 | Tanf_01245 knock-out cassette; ampRermFR | This work |
| pJET1.2/ΔTanf_01245+ | Cassette for reconstitution of ΔTanf_01245; ampRcatR | This work |
| pJET1.2/ΔTanf_01245+TFUB4_00887 | Cassette for cross-complementation of ΔTanf_01245 with TFUB4_00887; ampRcatR | This work |
| pJET1.2/ΔTFUB4_00887 | TFUB4_00887 knock-out cassette; ampRermFR | This work |
| pJET1.2/ΔTFUB4_00887+ | Cassette for reconstitution of ΔTFUB4_00887; ampRcatR | This work |
| pJET1.2/ΔTFUB4_00887+Tanf_01245 | Cassette for cross-complementation of ΔTFUB4_00887 with Tanf_01245; ampRcatR | This work |