Skip to main content
NCBI home page
Plant Physiology logoLink to Plant Physiology
. 1976 Apr;57(4):645–646. doi: 10.1104/pp.57.4.645

Aliphatic Chains of Esterified Lipids in Isolated Eyespots of Euglena gracilis var. bacillaris1

Lula L Hilenski 1,2, Patricia L Walne 1,2, Fred Snyder 1,2
PMCID: PMC542089  PMID: 16659543

Abstract

Isolated eyespot granules of Euglena gracilis Klebs var. bacillaris Pringsheim contained approximately 6% lipids (based on protein). Separation of the lipid extracts by thin layer chromatography revealed four major fractions: wax esters, triacylglycerols, free fatty acids, and phospholipids. Methanolysis of each fraction yielded between 27 and 29 different fatty acids ranging from 12:0 to 22:6. Acetates of the fatty alcohols of the wax fraction consisted of 11:0 to 18:0 carbon chains, with 14:0 being the major component; unsaturated alcohols were not detected.

Full text

PDF
645

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BLIGH E. G., DYER W. J. A rapid method of total lipid extraction and purification. Can J Biochem Physiol. 1959 Aug;37(8):911–917. doi: 10.1139/o59-099. [DOI] [PubMed] [Google Scholar]
  2. Bartlett C. J., Walne P. L., Schwarz O. J., Brown D. H. Large Scale Isolation and Purification of Eyespot Granules from Euglena gracilis var. bacillaris. Plant Physiol. 1972 Jun;49(6):881–885. doi: 10.1104/pp.49.6.881. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. GUEHLER P. F., PETERSON L., TSUCHIYA H. M., DODSON R. M. MICROBIOLOGICAL TRANSFORMATIONS. 13. COMPOSITION OF THE WAX FORMED BY EUGLENA GRACILIS. Arch Biochem Biophys. 1964 Jul 20;106:294–298. doi: 10.1016/0003-9861(64)90190-0. [DOI] [PubMed] [Google Scholar]
  4. Khan A. A., Kolattukudy P. E. Control of synthesis and distribution of acyl moieties in etiolated Euglena gracilis. Biochemistry. 1973 May 8;12(10):1939–1948. doi: 10.1021/bi00734a017. [DOI] [PubMed] [Google Scholar]
  5. Korn E. D. The fatty acids of Euglena gracilis. J Lipid Res. 1964 Jul;5(3):352–362. [PubMed] [Google Scholar]
  6. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  7. ROSENBERG A. A COMPARISON OF LIPID PATTERNS IN PHOTOSYNTHESIZING AND NONPHOTOSYNTHESIZING CELLS OF EUGLENA GRACILIS. Biochemistry. 1963 Sep-Oct;2:1148–1154. doi: 10.1021/bi00905a042. [DOI] [PubMed] [Google Scholar]
  8. Rosenberg A. Euglena gracilis: a novel lipid energy reserve and arachidonic acid enrichment during fasting. Science. 1967 Sep 8;157(3793):1189–1191. doi: 10.1126/science.157.3793.1189. [DOI] [PubMed] [Google Scholar]
  9. Rouser G., Siakotos A. N., Fleischer S. Quantitative analysis of phospholipids by thin-layer chromatography and phosphorus analysis of spots. Lipids. 1966 Jan;1(1):85–86. doi: 10.1007/BF02668129. [DOI] [PubMed] [Google Scholar]

Articles from Plant Physiology are provided here courtesy of Oxford University Press

RESOURCES