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. 1976 May;57(5):673–680. doi: 10.1104/pp.57.5.673

Alterations in Chloroplast Thylakoids during an in Vitro Freeze-Thaw Cycle 1

Melvin P Garber a,2, Peter L Steponkus a
PMCID: PMC542098  PMID: 16659550

Abstract

Plastocyanin and chloroplast coupling factor 1 (CF1) are released from spinach (Spinacia oleracea L.) thylakoids during a slow freezethaw cycle. CF1 addition increases the proton uptake of thylakoids previously frozen in sucrose concentrations of 15 mm to 100 mm. Addition of CF1 and plastocyanin restores the proton uptake of thylakoids frozen in 100 mm sucrose. Plastocyanin and CF1 release is a manifestation, not the cause, of freeze-thaw damage.

Frozen-thawed thylakoids appear to exhibit two levels of response to sucrose as measured by light-dependent proton uptake. Different levels of protection afforded by sucrose may be due, in part, to quantitative differences in CF1 release. The results suggest at least three freeze-induced lesions in light-dependent proton uptake by thylakoids: plastocyanin release, CF1 release, and disruption of the semi-permeability of thylakoids.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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