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. 1976 Jun;57(6):850–854. doi: 10.1104/pp.57.6.850

Auxin Biogenesis

Subcellular Compartmentation of Indoleacetaldehyde Reductases in Cucumber Seedlings 1

Peter J Bower a,2, Hugh M Brown a, William K Purves a
PMCID: PMC542134  PMID: 16659584

Abstract

Subcellular fractionation of cucumber (Cucumis sativus L.) seedlings was achieved, and two of the enzymes in the auxin biosynthetic pathway were localized. NADH-specific indoleacetaldehyde reductase activity was observed only in the cytosol fractions obtained from separated hypocotyl and cotyledon tissue. In contrast, a portion of the NADPH-specific indoleacetaldehyde reductase activity was associated with a microsomal fraction derived from these tissues. The NADPH-specific indoleacetaldehyde reductase was consistently found to be more firmly associated with the microsomal fraction derived from hypocotyls than with that from the cotyledons. These results indicate a division of the terminal steps of auxin biogenesis into at least two subcellular compartments.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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