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. 2017 Feb 6;36(25):3618–3628. doi: 10.1038/onc.2016.522

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Copyright © 2017 The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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EGF-induced activation of EGFR and phosphorylation of Picchu-X. (a) Schematic representation of conformational changes of the Picchu-X biosensor upon EGF stimulation and inhibition with TKI enabling FRET measurement of EGFR activity. (b) Effect of Mo-IPQA (10 μM) on EGF-induced (100 ng/ml) EGFR activation and biosensor phosphorylation in HCC1954 cells. (c) Effect of EGFR knockdown on phosphorylation of biosensor. (d) FRET/FLIM images of cells treated as in (b). Scale bar is 25 μm. (e) Quantification of FRET efficiencies for images in (d). The difference between untreated and EGF-treated cells is statistically significant (data are expressed as means±s.e.m.,*P<0.002, Student’s t-test, N>10 images).