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. 1976 Jul;58(1):28–32. doi: 10.1104/pp.58.1.28

Carbohydrate and Enzymic Characterization of a High Sucrose Sugary Inbred Line of Sweet Corn 1

Jorge W Gonzales a,2, Ashby M Rhodes a, David B Dickinson a
PMCID: PMC542173  PMID: 16659614

Abstract

Reserve carbohydrates were determined on developing endosperm of a new line of sugary maize (Zea mays L.). Other entries, included for comparative purposes, were Midway (sugary), Funks G4646 (starchy), and Illini X-tra Sweet (shrunken-2). Sucrose in the new line, Illinois 677a, was more than twice that of Midway at most stages of development, and reached a maximum of 40% of dry weight at 18 days after pollination. Appreciable phytoglycogen accumulated in Illinois 677a, reaching 30% or more of dry weight as endosperm tissue matured. Thus, Illinois 677a is a typical sugary maize concerning phytoglycogen content, but it resembles shrunken-2 concerning the extent of sucrose accumulation.

Enhanced sucrose accumulation by Illinois 677a was not accounted for by altered in vitro activities of invertase, sucrose synthase, UDP-glucose pyrophosphorylase, or ADP-glucose pyrophosphorylase. Its normal level of ADP-glucose pyrophosphorylase set Illinois 677a apart from shrunken-2 in which the enzyme was drastically reduced.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Amir J., Cherry J. H. Purification and properties of adenosine diphosphoglucose pyrophosphorylase from sweet corn. Plant Physiol. 1972 Jun;49(6):893–897. doi: 10.1104/pp.49.6.893. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Delmer D. P. The Regulatory Properties of Purified Phaseolus aureus Sucrose Synthetase. Plant Physiol. 1972 Oct;50(4):469–472. doi: 10.1104/pp.50.4.469. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Dickinson D. B., Preiss J. Presence of ADP-Glucose Pyrophosphorylase in Shrunken-2 and Brittle-2 Mutants of Maize Endosperm. Plant Physiol. 1969 Jul;44(7):1058–1062. doi: 10.1104/pp.44.7.1058. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Hodges H. F., Creech R. G., Loerch J. D. Biosynthesis of phytoglycogen in maize endosperm. The branching enzyme. Biochim Biophys Acta. 1969 Jul 8;185(1):70–79. doi: 10.1016/0005-2744(69)90283-6. [DOI] [PubMed] [Google Scholar]
  5. Hopper J. E., Dickinson D. B. Partial purification and sugar nucleotide inhibition of UDP-glucose pyrophosphorylase from Lilium longiflorum pollen. Arch Biochem Biophys. 1972 Feb;148(2):523–535. doi: 10.1016/0003-9861(72)90171-3. [DOI] [PubMed] [Google Scholar]
  6. Jaynes T. A., Nelson O. E. An invertase inactivator in maize endosperm and factors affecting inactivation. Plant Physiol. 1971 May;47(5):629–634. doi: 10.1104/pp.47.5.629. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Jaynes T. A., Nelson O. E. Invertase Activity in Normal and Mutant Maize Endosperms during Development. Plant Physiol. 1971 May;47(5):623–628. doi: 10.1104/pp.47.5.623. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Kidby D. K. Activation of a plant invertase by inorganic phosphate. Plant Physiol. 1966 Sep;41(7):1139–1144. doi: 10.1104/pp.41.7.1139. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  10. Laughnan J. R. The Effect of the sh(2) Factor on Carbohydrate Reserves in the Mature Endosperm of Maize. Genetics. 1953 Sep;38(5):485–499. doi: 10.1093/genetics/38.5.485. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Schiefer S., Lee E. Y.C., Whelan W. J. Multiple forms of starch synthetase in maize varieties as revealed by disc-gel electrophoresis and activity staining. FEBS Lett. 1973 Feb 15;30(1):129–132. doi: 10.1016/0014-5793(73)80634-9. [DOI] [PubMed] [Google Scholar]
  12. Shannon J. C., Creech R. G. Genetics of storage polyglucosides in Zea mays L. Ann N Y Acad Sci. 1973 Feb 9;210:279–289. doi: 10.1111/j.1749-6632.1973.tb47579.x. [DOI] [PubMed] [Google Scholar]

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