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Absorbance spectra of imidazoacridinone C-1375 (A) 10 μM, (B) 50 μM, (C) 65 μM in BSA-HA conjugate (mix) at 1 mg protein/ml (solid black line) compared to a mathematical summation (dotted line) of the absorbance spectra of pure C-1375 and pure conjugate solutions at the same concentrations as in the mix; (D) Delta Absorbance (mix –sum) at 444 nm at different C-1375 concentrations.
