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. 2017 Feb 15;8(15):25066–25079. doi: 10.18632/oncotarget.15339

Figure 4. Lower levels of CXCL16 in lungs of multiple KO mice.

Figure 4

A. B16F10 melanoma cells were injected i.v. into either WT or multiple KO mice. On day14 post injection, mice were sacrificed, blood and lungs were collected (n = 3-4/genotype), and protein array (detecting 62 mouse proteins; setup indicated below the panel) analysis was performed on serum samples and lung homogenates as indicated. Squares represent CXCL16; note the lower amounts of CXCL16 in samples from multiple KO mice. B. Mouse CXCL16 ELISA was performed on lung homogenates (n = 9-10/genotype) and serum (n = 4-6/genotype) from naïve mice (black bars) or mice injected with B16F10 melanoma cells (gray bars). Results are presented as mean ± SEM. C. qPCR analysis of CXCL16 gene expression was performed on lung tissue from naïve (black bars, n = 4) and B16F10 melanoma-injected mice (gray bars, n = 8). Expression of genes was evaluated relative to GAPDH and normalized to naïve mice. Results are presented as mean ± SEM (Kruskal-Wallis test). D. qPCR analysis of ADAM10 and ADAM17 expression was performed on lung tissue from naïve (black bars; n = 4-5) and B16F10 melanoma-injected mice (gray bars; n = 8). Expression of genes was evaluated relative to GAPDH and normalized to naïve mice. Results are presented as mean ± SEM. Statistical calculations were performed using the Mann Whitney test.