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. 1976 Aug;58(2):186–189. doi: 10.1104/pp.58.2.186

Photosynthetic Carbon Metabolism in the Palisade Parenchyma and Spongy Parenchyma of Vicia faba L. 1

William H Outlaw Jr a,2, Cheri L Schmuck a,3, N E Tolbert a
PMCID: PMC542209  PMID: 16659644

Abstract

Palisade parenchyma cells and spongy parenchyma cells were isolated separately from Vicia faba L. leaflets. Extracts of the cell isolates were assayed for several enzymes involved in CO2 fixation and photorespiration. When compared on a chlorophyll basis, the levels of enzyme activities either were equal in the different cell types or were greater in the spongy parenchyma; this difference is a reflection, perhaps, of the higher protein-chlorophyll ratio in the latter tissue. The distribution of radioactivity in the products of photosynthesis by each cell type was the same at various times after exposure to NaH14CO3, and the kinetics of 14C incorporation into these compounds was similar. However, a larger percentage of radioactivity was incorporated by the cell isolates into the 80% ethanol-insoluble fraction and correspondingly less into the neutral fraction as compared to whole leaf. It was concluded that photosynthetic CO2 fixation is similar in the different mesophyll tissues from which these cells were derived.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Andrews T. J., Lorimer G. H., Tolbert N. E. Ribulose diphosphate oxygenase. I. Synthesis of phosphoglycolate by fraction-1 protein of leaves. Biochemistry. 1973 Jan 2;12(1):11–18. doi: 10.1021/bi00725a003. [DOI] [PubMed] [Google Scholar]
  2. Bahr J. T., Jensen R. G. Ribulose bisphosphate oxygenase activity from freshly ruptured spinach chloroplasts. Arch Biochem Biophys. 1974 Oct;164(2):408–413. doi: 10.1016/0003-9861(74)90049-6. [DOI] [PubMed] [Google Scholar]
  3. Chen T. M., Dittrich P., Campbell W. H., Black C. C. Metabolism of epidermal tissues, mesophyll cells, and bundle sheath strands resolved from mature nutsedge leaves. Arch Biochem Biophys. 1974 Jul;163(1):246–262. doi: 10.1016/0003-9861(74)90475-5. [DOI] [PubMed] [Google Scholar]
  4. Frederick S. E., Newcomb E. H. Microbody-like organelles in leaf cells. Science. 1969 Mar 21;163(3873):1353–1355. doi: 10.1126/science.163.3873.1353. [DOI] [PubMed] [Google Scholar]
  5. Geiger P. J., Bessman S. P. Protein determination by Lowry's method in the presence of sulfhydryl reagents. Anal Biochem. 1972 Oct;49(2):467–473. doi: 10.1016/0003-2697(72)90450-2. [DOI] [PubMed] [Google Scholar]
  6. Gnanam A., Kulandaivelu G. Photosynthetic studies with leaf cell suspensions from higher plants. Plant Physiol. 1969 Oct;44(10):1451–1456. doi: 10.1104/pp.44.10.1451. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Jensen R. G., Francki R. I., Zaitlin M. Metabolism of separated leaf cells: I. Preparation of photosynthetically active cells from tobacco. Plant Physiol. 1971 Jul;48(1):9–13. doi: 10.1104/pp.48.1.9. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Kulandaivelu G., Gnanam A. Physiological studies with isolated leaf cells: early products of photosynthesis and their metabolic interconversions. Plant Physiol. 1974 Oct;54(4):569–574. doi: 10.1104/pp.54.4.569. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  10. Latzko E., Gibbs M. Enzyme activities of the carbon reduction cycle in some photosynthetic organisms. Plant Physiol. 1969 Feb;44(2):295–300. doi: 10.1104/pp.44.2.295. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Lilley R. M., Walker D. A. Carbon dioxide assimilation by leaves, isolated chloroplasts, and ribulose bisphosphate carboxylase from spinach. Plant Physiol. 1975 Jun;55(6):1087–1092. doi: 10.1104/pp.55.6.1087. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Miziorko H. M., Nowak T., Mildvan A. S. Spinach leaf phosphoenolpyruvate carboxylase: purification, properties, and kinetic studies. Arch Biochem Biophys. 1974 Jul;163(1):378–389. doi: 10.1016/0003-9861(74)90489-5. [DOI] [PubMed] [Google Scholar]
  13. Moss D. N., Rasmussen H. P. Cellular Localization of CO(2) Fixation and Translocation of Metabolites. Plant Physiol. 1969 Jul;44(7):1063–1068. doi: 10.1104/pp.44.7.1063. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Outlaw W. H., Fisher D. B. Compartmentation in Vicia faba Leaves: I. Kinetics of C in the Tissues following Pulse Labeling. Plant Physiol. 1975 Apr;55(4):699–703. doi: 10.1104/pp.55.4.699. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Randall D. D., Tolbert N. E. 3-Phosphoglycerate phosphatase in plants. I. Isolation and characterization from sugarcane leaves. J Biol Chem. 1971 Sep 10;246(17):5510–5517. [PubMed] [Google Scholar]
  16. Randall D. D., Tolbert N. E., Gremel D. 3-Phosphoglycerate Phosphatase in Plants: II. Distribution, Physiological Considerations, and Comparison with P-Glycolate Phosphatase. Plant Physiol. 1971 Oct;48(4):480–487. doi: 10.1104/pp.48.4.480. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Rehfeld D. W., Tolbert N. E. Aminotransferases in peroxisomes from spinach leaves. J Biol Chem. 1972 Aug 10;247(15):4803–4811. [PubMed] [Google Scholar]
  18. Tolbert N. E., Oeser A., Yamazaki R. K., Hageman R. H., Kisaki T. A survey of plants for leaf peroxisomes. Plant Physiol. 1969 Jan;44(1):135–147. doi: 10.1104/pp.44.1.135. [DOI] [PMC free article] [PubMed] [Google Scholar]

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