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. 1976 Sep;58(3):292–294. doi: 10.1104/pp.58.3.292

NADH- and NAD(P)H-Nitrate Reductases in Rice Seedlings

Teh-Chien Shen a,1, Edward A Funkhouser a, Miguel G Guerrero a
PMCID: PMC542233  PMID: 16659665

Abstract

By use of affinity chromatography on blue dextran-Sepharose, two nitrate reductases from rice (Oryza sativa L.) seedlings, specifically, NADH:nitrate oxidoreductase (EC 1.6.6.1) and NAD(P)-H:nitrate oxidoreductase (EC 1.6.6.2), have been partially separated. Nitrate-induced seedlings contained more NADH-nitrate reductase than NAD(P)H-nitrate reductase, whereas chloramphenicol-induced seedlings contained primarily NAD(P)H-nitrate reductase. NAD(P)H-nitrate reductase was shown to utilize NADPH directly as reductant. This enzyme has a preference for NADPH, but reacts about half as well with NADH.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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