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. 1976 Sep;58(3):324–330. doi: 10.1104/pp.58.3.324

Association of Latent Cellulase Activity with Plasma Membranes from Kidney Bean Abscission Zones 1

Don E Koehler a, Robert T Leonard a, William J Vanderwoude a, Arthur E Linkins a,2, Lowell N Lewis a
PMCID: PMC542240  PMID: 16659672

Abstract

Membranes isolated from abscission zones of Phaseolus vulgaris L., cv. Red Kidney, contained cellulase activity. This particulate activity was enhanced 10- to 20-fold by treatment with Triton X-100. Sucrose density gradient analyses of cell fractions showed that the membranes with which cellulase was associated had a peak equilibrium density of 1.16 to 1.17 g/cm3 which coincided with that of ion-activated ATPase, a marker for plasma membranes. The membrane fraction having the highest cellulase activity also contained a high proportion of plasma membranes as shown by electron microscopy of sucrose density gradient fractions after staining by periodic acid-chromic acid-phosphotungstic acid. It was concluded that the particulate cellulase was associated with the plasma membrane.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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