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. 1976 Sep;58(3):371–376. doi: 10.1104/pp.58.3.371

Correlation of Absorbance Changes and Thylakoid Fusion with the Induction of Oxygen Evolution in Bean Leaves Greened by Brief Flashes 1

Reto J Strasser a, Warren L Butler a
PMCID: PMC542249  PMID: 16659681

Abstract

Dark-grown bean leaves (Phaseolus vulgaris) which had been greened for several days in a repetitive series of brief xenon flashes were studied during the initial induction period when O2 evolution first appears. The induction of O2 evolution requires actinic irradiation (e.g. 2 mw/cm2 of red light) and goes to completion in about 8 minutes with a half-time just under 3 minutes. Absorbance measurements on the intact leaves showed that a change of a carotenoid pigment, monitored at 505 nm, was closely correlated with the rate of O2 evolution during the induction period. Inhibitor studies, however, showed that the absorbance change persisted in the presence of a number of inhibitors which blocked O2 evolution. Electron microscopy revealed that the primary thylakoids which were unfused in the flashed leaves before induction became fused in pairs or groups of three during the 8-minute induction period. It is postulated that the 505-nm absorbance change of the carotenoid pigment is correlated more directly with the fusion process than with O2 evolution. Heat treatment (45 C for 5 min) or infiltration with 0.8 m tris, which prevented the fusion process, also prevented the absorbance change.

If the leaves were preilluminated for 8 minutes with very weak red light (20 μw/cm2) which induced no O2 evolution, absorbance change, or thylakoid fusion, there was an immediate burst of O2 evolution at the onset of actinic irradiation and the induction period, as noted by O2 evolution or by the 505-nm absorbance change, was reduced to 2 minutes (half-time of 40 seconds). It is concluded that the electron transport system in the flashed leaves is blocked at the Mn site between water and photosystem II and that the photoactivation of Mn into the thylakoid membranes occurs during the low light, photoactivation process. After the electron transport chain is thus repaired, ion-pumping mechanisms driven by actinic light may lead to steady-state photosynthesis as well as to thylakoid fusion.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Butler W. L. Absorption spectroscopy of biological materials. Methods Enzymol. 1972;24:3–25. doi: 10.1016/0076-6879(72)24052-6. [DOI] [PubMed] [Google Scholar]
  2. Butler W. L., De Greef J., Roth T. F., Oelze-Karow H. The Influence of Carbonylcyanide-m-chlorophenylhydrazone and 3-(3,4-Dichlorophenyl)-1,1-dimethylurea on the Fusion of Primary Thylakoids and the Formation of Crystalline Fibrils in Bean Leaves Partially Greened in Far Red Light. Plant Physiol. 1972 Jan;49(1):102–104. doi: 10.1104/pp.49.1.102. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Cheniae G. M., Martin I. F. Photoreaction of manganese catalyst in photosynthetic oxygen evolution. Plant Physiol. 1969 Mar;44(3):351–360. doi: 10.1104/pp.44.3.351. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. De Greef J., Butler W. L., Roth T. F. Greening of etiolated bean leaves in far red light. Plant Physiol. 1971 Apr;47(4):457–464. doi: 10.1104/pp.47.4.457. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Hind G., Nakatani H. Y., Izawa S. Light-dependent redistribution of ions in suspensions of chloroplast thylakoid membranes. Proc Natl Acad Sci U S A. 1974 Apr;71(4):1484–1488. doi: 10.1073/pnas.71.4.1484. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Izawa S., Good N. E. Effect of salts and electron transport on the conformation of isolated chloroplasts. I. Light-scattering and volume changes. Plant Physiol. 1966 Mar;41(3):533–543. doi: 10.1104/pp.41.3.533. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Murakami S., Packer L. The role of cations in the organization of chloroplast membranes. Arch Biochem Biophys. 1971 Sep;146(1):337–347. doi: 10.1016/s0003-9861(71)80072-3. [DOI] [PubMed] [Google Scholar]
  8. Oelze-Karow H., Butler W. L. The development of photophosphorylation and photosynthesis in greening bean leaves. Plant Physiol. 1971 Nov;48(5):621–625. doi: 10.1104/pp.48.5.621. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Strasser R. J. A new device for timultaneous measurements of oxygen concentration. Absorption and fluorescence changes in photosynthetic systems. Experientia. 1974 Mar 15;30(3):320–320. doi: 10.1007/BF01934855. [DOI] [PubMed] [Google Scholar]
  10. Strasser R. J. Induction phenomena in green plants when the photosynthetic apparatus starts to work. Arch Int Physiol Biochim. 1973 Dec;81(5):935–955. [PubMed] [Google Scholar]
  11. Strasser R. J., Sironval C. Induction of PS II activity and induction of a variable part of the fluorescence emission by weak green light in flashed bean leaves. FEBS Lett. 1973 Feb 1;29(3):286–288. doi: 10.1016/0014-5793(73)80040-7. [DOI] [PubMed] [Google Scholar]

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