Skip to main content
NCBI home page
Plant Physiology logoLink to Plant Physiology
. 1976 Nov;58(5):626–630. doi: 10.1104/pp.58.5.626

Dormancy in Seeds of Charlock (Sinapis arvensis L.)

Early Effects of Gibberellic Acid on the Synthesis of Amino Acids and Proteins 1

Miriam Edwards a
PMCID: PMC542271  PMID: 16659732

Abstract

Charlock (Sinapis arvensis L.) seeds were imbibed with 10 mm GA3 for 24 hours at 0 C. After equilibration at 25 C, a 5-fold increase in radioactivity in the amino acids labeled from 2-14C-acetate was observed within 2 hours. The total amount of amino acids was reduced to half, and the specific radioactivity increased approximately 10-fold, indicating a diversion of metabolites for amino acid and protein synthesis in GA3-treated seeds. The rate of incorporation of l-14 C-leucine into protein was doubled. Autoradiographs showed that enhancement of protein synthesis was localized in the shoot and root meristems, the developing vascular tissues, and in the endosperm cells inside the testa.

Full text

PDF
626

Images in this article

628Image
on p.628
629Image
on p.629

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. CANVIN D. T., BEEVERS H. Sucrose synthesis from acetate in the germinating castor bean: kinetics and pathway. J Biol Chem. 1961 Apr;236:988–995. [PubMed] [Google Scholar]
  2. Chen S. S., Chang J. L. Does Gibberellic Acid Stimulate Seed Germination via Amylase Synthesis? Plant Physiol. 1972 Mar;49(3):441–442. doi: 10.1104/pp.49.3.441. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Chen S. S., Varner J. E. Respiration and Protein Synthesis in Dormant and After-ripened Seeds of Avena fatua. Plant Physiol. 1970 Jul;46(1):108–112. doi: 10.1104/pp.46.1.108. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Jarvis B. C., Frankland B., Cherry J. H. Increased DNA template and RNA polymerase associated with the breaking of seed dormancy. Plant Physiol. 1968 Oct;43(10):1734–1736. doi: 10.1104/pp.43.10.1734. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Tuan D. Y., Bonner J. Dormancy Associated with Repression of Genetic Activity. Plant Physiol. 1964 Sep;39(5):768–772. doi: 10.1104/pp.39.5.768. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. WERBIN H., CHAIKOFF I. L., IMADA M. R. Rapid sensitive method for determining H3-water in body fluids by liquid scintillation spectrometry. Proc Soc Exp Biol Med. 1959 Oct;102:8–12. doi: 10.3181/00379727-102-25125. [DOI] [PubMed] [Google Scholar]

Articles from Plant Physiology are provided here courtesy of Oxford University Press

RESOURCES