Skip to main content
NCBI home page
Plant Physiology logoLink to Plant Physiology
. 1976 Nov;58(5):666–669. doi: 10.1104/pp.58.5.666

Metabolism of Indole-3-Acetic Acid

III. Identification of Metabolites Isolated from Crown Gall Callus Tissue 1

Chao-Shieung Feung a,2, Robert H Hamilton a, Ralph O Mumma a
PMCID: PMC542279  PMID: 16659740

Abstract

The metabolism of labeled indole-3-acetic acid (IAA-2-14C) was investigated in Parthenocissus tricuspidata crown gall callus tissue. After 48 hours incubation, 85 to 90% of the supplied IAA was taken up by the tissue, and of that taken up, about 45% was conjugated with five amino acids. The conjugates found were aspartic and glutamic acid (minor ones) as well as glycine, alanine, and valine (major ones). The last four are being reported for the first time as metabolites of IAA. These conjugates were identified through their chromatographic properties, hydrolysis products, and their mass spectra. The possible significance of these amino acid conjugates is discussed.

Full text

PDF
666

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Andreae W. A., Good N. E. The Formation of Indoleacetylaspartic Acid in Pea Seedlings. Plant Physiol. 1955 Jul;30(4):380–382. doi: 10.1104/pp.30.4.380. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Andreae W. A., Van Ysselstein M. W. Studies on 3-Indoleacetic Acid Metabolism. VI. 3-Indoleacetic Acid Uptake and Metabolism by Pea Roots and Epicotyls. Plant Physiol. 1960 Mar;35(2):225–232. doi: 10.1104/pp.35.2.225. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Andreae W. A., Ysselstein M. W. Studies on 3-Indoleacetic Acid Metabolism. III. The Uptake of 3-Indoleacetic Acid by Pea Epicotyls and Its Conversion to 3-Indoleacetylaspartic Acid. Plant Physiol. 1956 May;31(3):235–240. doi: 10.1104/pp.31.3.235. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Beyer E. M., Morgan P. W. Effect of ethylene on the uptake, distribution, and metabolism of indoleacetic Acid-1-C and -2-C and naphthaleneacetic Acid-1-C. Plant Physiol. 1970 Jul;46(1):157–162. doi: 10.1104/pp.46.1.157. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Feung C., Hamilton R. H., Mumma R. O. Metabolism of 2,4-dichlorophenoxyacetic acid. V. Identification of metabolites in soybean callus tissue cultures. J Agric Food Chem. 1973 Jul-Aug;21(4):637–640. doi: 10.1021/jf60188a058. [DOI] [PubMed] [Google Scholar]
  6. Good N. E., Andreae W. A., Ysselstein M. W. Studies on 3-Indoleacetic Acid Metabolism. II. Some Products of the Metabolism of Exogenous Indoleacetic Acid in Plant Tissues. Plant Physiol. 1956 May;31(3):231–235. doi: 10.1104/pp.31.3.231. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Goren R., Bukovac M. J., Flore J. A. Mechanism of Indole-3-acetic Acid Conjugation: No Induction by Ethylene. Plant Physiol. 1974 Feb;53(2):164–166. doi: 10.1104/pp.53.2.164. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Hamilton R. H., Meyer H. E., Burke R. E., Feung C. S., Mumma R. O. Metabolism of Indole-3-Acetic Acid: II. Oxindole Pathway in Parthenocissus tricuspidata Crown-Gall Tissue Cultures. Plant Physiol. 1976 Jul;58(1):77–81. doi: 10.1104/pp.58.1.77. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Hutzinger O., Kosuge T. Microbial synthesis and degradation of indole-3-acetic acid. 3. The isolation and characterization of indole-3-acetyl-epsilon-L-lysine. Biochemistry. 1968 Feb;7(2):601–605. doi: 10.1021/bi00842a013. [DOI] [PubMed] [Google Scholar]
  10. Lau O. L., Murr D. P., Yang S. F. Effect of 2,4-Dinitrophenol on Auxin-induced Ethylene Production and Auxin Conjugation by Mung Bean Tissue. Plant Physiol. 1974 Aug;54(2):182–185. doi: 10.1104/pp.54.2.182. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Olney H. O. Growth substances from Veratrum tenuipetalum. Plant Physiol. 1968 Mar;43(3):293–302. doi: 10.1104/pp.43.3.293. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Robinson B. J., Forman M., Addicott F. T. Auxin transport and conjugation in cotton explants. Plant Physiol. 1968 Aug;43(8):1321–1323. doi: 10.1104/pp.43.8.1321. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Venis M. A. Auxin-induced Conjugation Systems in Peas. Plant Physiol. 1972 Jan;49(1):24–27. doi: 10.1104/pp.49.1.24. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Winter A., Thimann K. V. Bound indoleacetic Acid in Avena coleoptiles. Plant Physiol. 1966 Feb;41(2):335–342. doi: 10.1104/pp.41.2.335. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. ZENK M. H. I-(Indole-3-acetyl)-beta-D-glucose, a new compound in the metabolism of indole-3-acetic acid in plants. Nature. 1961 Jul 29;191:493–494. doi: 10.1038/191493a0. [DOI] [PubMed] [Google Scholar]

Articles from Plant Physiology are provided here courtesy of Oxford University Press

RESOURCES