Abstract
Wild carrot (Daucus carota L.) cell suspensions were grown on a mineral salt medium supplemented with 10 mmmyoinositol in the presence and absence of 2.25 μm 2,4-dichlorophenoxyacetic acid (2,4-D), and a variety of carbon sources.
The data obtained on growth and embryo number in the absence of 2,4-D show that wild carrot suspensions were able to utilize sucrose, glucose, fructose, galactose, mannose, maltose, raffinose, or stachyose as a carbon source. A highly significant correlation between dry weight and embryo number was obtained regardless of the carbohydrate source suggesting the involvement of a common intermediate in the metabolism of the various sugars.
In the presence of 2.25 μm 2,4-D, embryo formation was suppressed. Time course of dry weights obtained in the presence and absence of 2,4-D show that 2,4-D increased the growth rate of the tissue when glucose, fructose, mannose, or stachyose was used as the carbon source. The growth rates on other sugars remained unchanged under these conditions.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Roberts R. M., Heishman A., Wicklin C. Growth Inhibition and Metabolite Pool Levels in Plant Tissues Fed d-Glucosamine and d-Galactose. Plant Physiol. 1971 Jul;48(1):36–42. doi: 10.1104/pp.48.1.36. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Stehsel M. L., Caplin S. M. Sugars: autoclaving vs sterile filtration on the growth of carrot root tissue in culture. Life Sci. 1969 Dec 15;8(24):1255–1259. doi: 10.1016/0024-3205(69)90182-9. [DOI] [PubMed] [Google Scholar]
- Wetherell D. F. Phytochrome in cultured wild carrot tissue. I. Synthesis. Plant Physiol. 1969 Dec;44(12):1734–1737. doi: 10.1104/pp.44.12.1734. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Wright K., Northcote D. H. Induced root differentiation in sycamore callus. J Cell Sci. 1972 Sep;11(2):319–337. doi: 10.1242/jcs.11.2.319. [DOI] [PubMed] [Google Scholar]
- Zenk M. H., el-Shagi H., Schulte U. Anthraquinone production by cell suspension cultures of Morinda citrifolia. Planta Med. 1975;Suppl:79–101. doi: 10.1055/s-0028-1104768. [DOI] [PubMed] [Google Scholar]