Abstract
Nitrite reduction in either whole, isolated spinach chloroplasts (Spinacia oleracea L.) or in reconstituted spinach chloroplasts is stimulated by a short period of photosynthetic CO2 fixation in the light prior to nitrite addition. With reconstituted chloroplasts, a similar stimulation can be obtained in nitrite reduction without CO2 fixation by the addition of dihydroxyacetone phosphate or fructose 6-phosphate. Specific intermediate metabolites of the photosynthetic carbon reduction cycle may have a regulatory role in nitrite reduction in chloroplasts in the light.
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- Dalling M. J., Tolbert N. E., Hageman R. H. Intracellular location of nitrate reductase and nitrite reductase. I. Spinach and tobacco leaves. Biochim Biophys Acta. 1972 Dec 14;283(3):505–512. doi: 10.1016/0005-2728(72)90266-6. [DOI] [PubMed] [Google Scholar]
- Jensen R. G., Bassham J. A. Photosynthesis by isolated chloroplasts. Proc Natl Acad Sci U S A. 1966 Oct;56(4):1095–1101. doi: 10.1073/pnas.56.4.1095. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kanazawa T., Kirk M. R., Bassham J. A. Regulatory effects of ammonia on carbon metabolism in photosynthesizing Chlorella pyrenoidosa. Biochim Biophys Acta. 1970 Jun 30;205(3):401–408. doi: 10.1016/0005-2728(70)90106-4. [DOI] [PubMed] [Google Scholar]
- Losada M., Ramírez J. M., Paneque A., Del Campo F. F. Light and dark reduction of nitrate in a reconstituted chloroplast system. Biochim Biophys Acta. 1965 Sep 27;109(1):86–96. doi: 10.1016/0926-6585(65)90093-2. [DOI] [PubMed] [Google Scholar]
- MEDINA A., NICHOLAS D. J. Interference by reduced pyridine nucleotides in the diazotization of nitrite. Biochim Biophys Acta. 1957 Feb;23(2):440–442. doi: 10.1016/0006-3002(57)90355-4. [DOI] [PubMed] [Google Scholar]
- Magalhaes A. C., Neyra C. A., Hageman R. H. Nitrite assimilation and amino nitrogen synthesis in isolated spinach chloroplasts. Plant Physiol. 1974 Mar;53(3):411–415. doi: 10.1104/pp.53.3.411. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Mitchell C. A., Stocking C. R. Kinetics and Energetics of Light-driven Chloroplast Glutamine Synthesis. Plant Physiol. 1975 Jan;55(1):59–63. doi: 10.1104/pp.55.1.59. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Neyra C. A., Hageman R. H. Dependence of nitrite reduction on electron transport chloroplasts. Plant Physiol. 1974 Oct;54(4):480–483. doi: 10.1104/pp.54.4.480. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ritenour G. L., Joy K. W., Bunning J., Hageman R. H. Intracellular localization of nitrate reductase, nitrite reductase, and glutamic Acid dehydrogenase in green leaf tissue. Plant Physiol. 1967 Feb;42(2):233–237. doi: 10.1104/pp.42.2.233. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sanderson G. W., Cocking E. C. Enzymic Assimilation of Nitrate in Tomato Plants. I. Reduction of Nitrate to Nitrite. Plant Physiol. 1964 May;39(3):416–422. doi: 10.1104/pp.39.3.416. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Scholl R. L., Harper J. E., Hageman R. H. Improvements of the nitrite color development in assays of nitrate reductase by phenazine methosulfate and zinc acetate. Plant Physiol. 1974 Jun;53(6):825–828. doi: 10.1104/pp.53.6.825. [DOI] [PMC free article] [PubMed] [Google Scholar]