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. 1977 Mar;59(3):365–368. doi: 10.1104/pp.59.3.365

Effects of Osmotic Shock on Some Membrane-regulated Events of Oat Coleoptile Cells 1

Bernard Rubinstein a, Peter Mahar a, Terry A Tattar b
PMCID: PMC542403  PMID: 16659852

Abstract

Oat coleoptile sections (Avena sativa L. cv. “Garry”) were osmotically shocked with 0.5 m mannitol followed by 1 mm Na-phosphate (pH 6.4) at 4 C. This treatment reduced uptake of α-aminoisobutyric acid, 3-o-methyl glucose, and leucine by 75 to 90% but inhibited 36Cl− uptake only 30%. Some recovery was observed 1 to 3 hours later. Respiration rates were unaffected by osmotic shock and protein synthesis was reduced 11%.

Osmotic shock also stimulated efflux of α-aminoisobutyric acid and K+ and led to an increase in conductivity of the solution bathing shocked sections. The transmembrane electropotential of 75% of the shocked cells fell to −20 mv to −45 mv compared with the majority of unshocked cells at −80 mv to −120 mv.

We concluded that osmotic shock selectively modifies the plasma membrane. The inhibitions of uptake could be due to removal of specific components of the plasma membrane and/or to the lowered electropotential.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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