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. 2014 Feb 24;3(2):e89. doi: 10.1038/oncsis.2014.2

Figure 3.

Figure 3

Knockdown of TNIK by siRNA led to cell growth inhibition and death. (a, b) siRNAs against TNIK and scramble sequence were introduced in cells by transfection with HiPerFect. Cell proliferation (a) and death (b) were monitored by Acumen analysis after transfection for 96 h. (c) Knockdown of TNIK protein expression by TNIK siRNAs. Cells were transfected with different TNIK siRNAs or scrambled siRNA for 48 h, and cell lysates were collected for western blotting analysis using anti-TNIK antibody. The data represented here are representative from three independent experiments. UN, untreated cells, NC, scrambled control, S2, TNIKsiRNA 2, S3, TNIKsiRNA3. *P<0.05, **P<0.01.