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. 1977 Mar;59(3):459–463. doi: 10.1104/pp.59.3.459

Phosphatidic Acid Synthesis in Castor Bean Endosperm 1

Brady Vick a, Harry Beevers a
PMCID: PMC542424  PMID: 16659873

Abstract

Enzyme assays on organelles isolated from the endosperm of castor bean (Ricinus communis var. Hale) by sucrose density gradient centrifugation showed that palmitoyl-CoA:sn-glycerol 3-phosphate acyltransferase (EC 2.3.1.15) was localized in the membranes of the endoplasmic reticulum. Mn2+ was required for activity, but Ca2+ and Mg2+ could substitute for Mn2+ at higher concentrations. The apparent Km was 170 μm for sn-glycerol 3-phosphate and approximately 8 μm for palmitoyl-CoA. The optimum pH range was 7 to 7.5 and the principal reaction product was diacyl-sn-glycerol 3-phosphate (phosphatidic acid). Monoacyl-sn-glycerol 3-phosphate (lysophosphatidic acid) was not released as a free intermediate in the reaction. The maximum activity of the enzyme occurred immediately after imbibition, preceding the development of mitochondria and glyoxysomes.

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Selected References

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