Abstract
A phytostat to mass culture higher plant cells in liquid medium is described. This apparatus allowed the culture in batch, turbidostat and chemostat of 20 liters of cells. Automatic control of cell suspension growth was based on culture turbidity. Changes with time of certain cell characteristics, particularly cell respiration and phospholipid content, indicated that the test time to harvest large amounts of sycamore cells (Acer pseudoplatanus L.) in good physiological state was about 2 days before the end of the exponential phase of growth, when the cell density reached one million cells per milliliter of culture.
Full text
PDFImages in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- FOLCH J., LEES M., SLOANE STANLEY G. H. A simple method for the isolation and purification of total lipides from animal tissues. J Biol Chem. 1957 May;226(1):497–509. [PubMed] [Google Scholar]
- LAMPORT D. T. CELL SUSPENSION CULTURES OF HIGHER PLANTS: ISOLATION AND GROWTH ENERGETICS. Exp Cell Res. 1964 Jan;33:195–206. doi: 10.1016/s0014-4827(64)81026-0. [DOI] [PubMed] [Google Scholar]
- Leguay J. J., Guern J. Quantitative Effects of 2,4-Dichlorophenoxyacetic Acid on Growth of Suspension-cultured Acer pseudoplatanus Cells. Plant Physiol. 1975 Sep;56(3):356–359. doi: 10.1104/pp.56.3.356. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sung Z. R. Turbidimetric measurement of plant cell culture growth. Plant Physiol. 1976 Mar;57(3):460–462. doi: 10.1104/pp.57.3.460. [DOI] [PMC free article] [PubMed] [Google Scholar]
- TAUSSKY H. H., SHORR E. A microcolorimetric method for the determination of inorganic phosphorus. J Biol Chem. 1953 Jun;202(2):675–685. [PubMed] [Google Scholar]
- Torrey J. G., Reinert J. Suspension cultures of higher plant cells in synthetic media. Plant Physiol. 1961 Jul;36(4):483–491. doi: 10.1104/pp.36.4.483. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Tulecke W. Continuous cultures of higher plant cells in liquid media: the advantages and potential use of a phytostat. Ann N Y Acad Sci. 1966 Oct 7;139(1):162–175. doi: 10.1111/j.1749-6632.1966.tb41193.x. [DOI] [PubMed] [Google Scholar]