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. 1977 Apr;59(4):525–529. doi: 10.1104/pp.59.4.525

Storage Protein Synthesis in Maize

II. Reduced Synthesis of a Major Zein Component by the Opaque-2 Mutant of Maize 1,2

Richard A Jones a,3, Brian A Larkins a, C Y Tsai a
PMCID: PMC542441  PMID: 16659886

Abstract

Two zein proteins (Z1 and Z2) represent the majority of the protein synthesized during maize endosperm development. Undegraded membrane-bound polysomes isolated from normal maize synthesized these proteins when incubated in a cell-free protein-synthesizing system from wheat germ. The proteins synthesized in vitro were similar to authentic zein in ethanol solubility and electrophoretic mobility. Zein synthesis was associated with large size classes of membrane bound polysomes in normal maize.

Membrane-bound polysomes isolated from developing kernels of opaque-2 mutant synthesized less total zein in vitro, and dramatically reduced incorporation into the Z1 component. The reduction in total zein corresponded to a 50% reduction in the level of membrane-bound polysomes in opaque-2, and the near absence of the large polysome size classes, which synthesized zein in normal maize. We concluded that the opaque-2 mutation results in a decreased “availability” of the zein mRNAs, reflected in a reduced level of membrane-bound polysomes.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BRUNNGRABER E. F. A simplified procedure for the preparation of "soluble" RNA from rat liver. Biochem Biophys Res Commun. 1962 Jun 19;8:1–3. doi: 10.1016/0006-291x(62)90223-1. [DOI] [PubMed] [Google Scholar]
  2. Davies E., Larkins B. A. Polyribosome degradation as a sensitive assay for endolytic messenger-ribonuclease activity. Anal Biochem. 1974 Sep;61(1):155–164. doi: 10.1016/0003-2697(74)90342-x. [DOI] [PubMed] [Google Scholar]
  3. GOODMAN H. M., RICH A. MECHANISM OF POLYRIBOSOME ACTION DURING PROTEIN SYNTHESIS. Nature. 1963 Jul 27;199:318–322. doi: 10.1038/199318a0. [DOI] [PubMed] [Google Scholar]
  4. Jones R. A., Larkins B. A., Tsai C. Y. Reduced synthesis of zein in vitro by a high lysine mutant of maize. Biochem Biophys Res Commun. 1976 Mar 22;69(2):404–410. doi: 10.1016/0006-291x(76)90536-2. [DOI] [PubMed] [Google Scholar]
  5. Larkins B. A., Bracker C. E., Tsai C. Y. Storage Protein Synthesis in Maize: Isolation of Zein-synthesizing Polyribosomes. Plant Physiol. 1976 May;57(5):740–745. doi: 10.1104/pp.57.5.740. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Larkins B. A., Dalby A. In vitro synthesis of zein-like protein by maize polyribosomes. Biochem Biophys Res Commun. 1975 Oct 6;66(3):1048–1054. doi: 10.1016/0006-291x(75)90746-9. [DOI] [PubMed] [Google Scholar]
  7. Larkins B. A., Davies E. Polyribosomes from Peas: V. An Attempt to Characterize the Total Free and Membrane-bound Polysomal Population. Plant Physiol. 1975 Apr;55(4):749–756. doi: 10.1104/pp.55.4.749. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Lee K. H., Jones R. A., Dalby A., Tsai C. Y. Genetic regulation of storaage protein content in maize endosperm. Biochem Genet. 1976 Aug;14(7-8):641–650. doi: 10.1007/BF00485842. [DOI] [PubMed] [Google Scholar]
  9. MERTZ E. T., BATES L. S., NELSON O. E. MUTANT GENE THAT CHANGES PROTEIN COMPOSITION AND INCREASES LYSINE CONTENT OF MAIZE ENDOSPERM. Science. 1964 Jul 17;145(3629):279–280. doi: 10.1126/science.145.3629.279. [DOI] [PubMed] [Google Scholar]
  10. Marcus A., Efron D., Weeks D. P. The wheat embryo cell-free system. Methods Enzymol. 1974;30:749–754. doi: 10.1016/0076-6879(74)30073-0. [DOI] [PubMed] [Google Scholar]
  11. Mertz E. T., Veron O. A., Bates L. S., Nelson O. E. Growth of Rats Fed on Opaque-2 Maize. Science. 1965 Jun 25;148(3678):1741–1742. doi: 10.1126/science.148.3678.1741. [DOI] [PubMed] [Google Scholar]
  12. Nelson O. E., Mertz E. T., Bates L. S. Second Mutant Gene Affecting the Amino Acid Pattern of Maize Endosperm Proteins. Science. 1965 Dec 10;150(3702):1469–1470. doi: 10.1126/science.150.3702.1469. [DOI] [PubMed] [Google Scholar]
  13. Vassart G., Dumont J. E. Identification of polysomes synthesizing thyroglobulin. Eur J Biochem. 1973 Jan 15;32(2):322–330. doi: 10.1111/j.1432-1033.1973.tb02613.x. [DOI] [PubMed] [Google Scholar]
  14. Verma D. P., Nash D. T., Schulman H. M. Isolation and in vitro translation of soybean leghaemoglobin mRNA. Nature. 1974 Sep 6;251(5470):74–77. doi: 10.1038/251074a0. [DOI] [PubMed] [Google Scholar]

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